In vitro antibacterial activity of different endodontic irrigants

Department of Operative Dentistry, University of Pavia, Piazzale Golgi 3, Pavia, Italy.
Dental Traumatology (Impact Factor: 1.6). 11/2011; 28(3):205-9. DOI: 10.1111/j.1600-9657.2011.01074.x
Source: PubMed


The objective of this study was to compare in vitro the antibacterial activity of Tetraclean (mixture of doxycycline, citric acid and polypropylene glycol), Niclor 5 (5.25% sodium hypochlorite solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution) and hydrogen peroxide 12 volumes on three endodontic pathogens associated with primary endodontic infections. Enterococcus faecalis, Streptococcus mutans and Staphylococcus aureus strains were selected to evaluate the antibacterial activity of endodontic irrigants by the agar disc-diffusion test.
Paper discs were saturated with each one of the test solutions (at room temperature and preheating at 50°C) and placed onto culture agar-plates preadsorbed with bacterial cells and further incubated for 24 h at 37°C. The growth inhibition zones around each irrigant were recorded and compared for each bacterial strain. Kruskal-Wallis and Mann-Whitney tests were applied to compare the various groups.
At room temperature, Tetraclean showed significantly higher inhibition of bacteria growth than all other irrigants tested. Preheating at 50°C significantly increased growth inhibition for all the groups tested. At 50°C, hydrogen peroxide 12 volumes and Tetraclean showed significantly higher efficacy than all other irrigants tested.
50°C-preheated hydrogen peroxide 12 volumes and Tetraclean showed highest inhibition of the bacterial growth.

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    • "These authors employed confocal microscopy to evaluate bacterial cell viability after the exposure of oral bacteria biofilm to NaOCl and CHX, and showed that NaOCl was pronouncedly more effective than CHX to kill bacteria cells organized in biofilm (Ordinola-Zapata et al., 2012). Other studies that have compared the effectiveness of NaOCl and CHX against E. faecalis showed that CHX was as effective as, or even more effective than NaOCl, but these studies did not use a method based on antimicrobial activity against biofilms (Vianna and Gomes, 2009), or used unreliable methods such as the agar diffusion test (Poggio et al., 2012; Mattigatti et al., 2012; Sassone et al., 2008). However, it has been clearly shown that bacteria are usually organized in biofilm structures or within necrotic pulp tissue remnants in the root canal system (Baldasso et al., 2012; Vera et al., 2012; Ricucci and Siqueira, 2010; Ricucci et al., 2009), and these tissues/structures may protect bacterial cells against antimicrobial action. "
    10/2014; 6(6):64-69. DOI:10.5897/JDOH2014.0128
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    • "Agar disc-diffusion test method is a well-established technique for antibacterial study.[1011] Advantage of this method is that chemical properties of the medicaments are not changed and the antimicrobial resistance can be detected by challenging bacterial isolates with antimicrobial discs.[12] "
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