Article

Correlation between expression of cellular retinol-binding protein 1 and its methylation status in larynx cancer

Laboratorio de Oncogenomica, UIMEO, Hospital de Oncología, CMN-SXXI, IMSS, México, Distrito Federal, Mexico.
Journal of clinical pathology (Impact Factor: 2.55). 11/2011; 65(1):46-50. DOI: 10.1136/jclinpath-2011-200304
Source: PubMed

ABSTRACT The authors have previously reported that cellular retinol-binding protein 1 (CRBP1) gene gain and its expression correlated significantly with survival in laryngeal carcinoma patients. The authors hypothesised that inactivation of the CRBP1 gene through CpG methylation is associated with patient status and gene expression. In this work, the authors determine the expression and methylation status of the CRBP1 gene and its correlation with clinical variables of laryngeal carcinoma patients.
The CRBP1 gene methylation promoter was assessed by methylation specific PCR analysis in tissue samples from larynx cancer specimens and its expression was assessed by immunohistochemistry on paraffin embedded tissue using tissue microarray. The results were then compared with the clinical pathological variables and outcome measures. The study included 46 samples from patients with non-metastatic squamous cell carcinoma of the larynx without any previous oncological treatments.
Lack of CRBP1 expression was seen in 17 of the 46 laryngeal carcinoma samples, while the remaining 29 samples showed increased expression. Significant associations were found between CRBP1 expression and methylation and patient status. There was a tendency for association in all clinical stages of the disease. CRBP1 gene expression and its unmethylated promoter correlated significantly with survival (p<0.05).
An early event of larynx cancer could be CRBP1 expression related to unmethylation of the promoter region. These features could also be associated with good response and survival. The authors hypothesised that increased expression and unmethylated promoter of the CRBP1 gene could be considered as markers for larynx cancer.

Full-text

Available from: Raúl Peralta-Rodríguez, Jul 17, 2014
2 Followers
 · 
100 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Hypermethylated in cancer 1 (HIC1) is a putative suppressor gene, cooperating with TP53 in the regulation of apoptosis. The promoter site of this gene contains CpG islands susceptible to methylation. Altered methylation leads to the silencing of HIC1. Persistent loss of HIC1 function reflects the attenuation of proapoptotic characteristics of TP53 and may constitute the background for carcinogenesis. Altered methyla‑ tion profiles along with diminished expression of HIC1 were documented in a number of solid neoplasms. The aim of this study was to evaluate the expression of the HIC1 gene in laryn‑ geal carcinoma. RNA was extracted from samples of laryngeal cancer and corresponding healthy tissues of 21 patients with advanced laryngeal cancer (T3‑T4). The amount of RNA (cDNA) was evaluated using reverse transcription‑quantitative polymerase chain reaction with GADPH as the reference gene. Data demonstrated that HIC1 expression was significantly reduced in laryngeal cancer tissues. The relative expression of HIC1 was found to be ~40% lower in tumor samples compared to that in healthy controls. The median tumor/normal tissue ratio for HIC1 was 0.615. These results suggest that low HIC1 expression may be associated with neoplastic transformation in the larynx.
    Oncology letters 02/2015; 149(202). DOI:10.3892/ol.2015.2983 · 0.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Increasing evidence indicates the high heterogeneity of cancer cells. Recent studies have revealed distinct subtypes of DNA methylation in colorectal cancer (CRC); however, the mechanism of heterogeneous methylation remains poorly understood. Gene expression is a natural, intermediate quantitative trait that bridges genotypic and phenotypic features. In this work, we studied the role of heterogeneous DNA methylation in tumorigenesis via gene expression analyses. Specifically, we integrated methylation and expression data in normal and tumor tissues, and examined the perturbations in coexpression patterns. We found that the heterogeneity of methylation leads to significant loss of coexpression connectivity in CRC; this finding was validated in an independent cohort. Functional analyses showed that the lost coexpression partners participate in important cancer-related pathways/networks, such as ErbB and mitogen-activated protein kinase (MAPK) signaling pathways. Our analyses suggest that the loss of coexpression connectivity induced by methylation heterogeneity might play an important role in CRC. To our knowledge, this is the first study to interpret methylation heterogeneity in cancer from the perspective of coexpression perturbation. Our results provide new perspectives in tumor biology and may facilitate the identification of potential biomedical therapies for cancer treatment. © 2014 Wiley Periodicals, Inc. Copyright © 2014 Wiley Periodicals, Inc.
    Genes Chromosomes and Cancer 11/2014; 54(2). DOI:10.1002/gcc.22224 · 3.84 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we quantified the expression of CRBP1 and CRBP3 in Roman layer (R) and Erlang mountainous chickens (SD02 and SD03), to discern the tissue, breed and age-related expression patterns in order to discover potential involvement in egg production and other related reproduction traits. Real-time quantitative PCR assays were developed for accurate measurement of CRBP1 and CRBP3 mRNA levels in different tissues from chickens at four ages (12, 20, 32 and 45 weeks). We found that the CRBP1 and CRBP3 were expressed in all six tissues examined in all three breeds of chicken at 32 weeks. CRBP1 mRNA levels in SD02 kidneys were slightly higher than those in SD03 and R at 12 weeks, whereas, at the other three time points, the expression levels of CRBP1 in SD03 were higher than those in SD02 and R. In addition, there was higher hepatic expression of CRBP3 mRNA in layers (R) compared to broilers (SD02 and SD03) at 20 and 32 weeks. An age-related expression pattern of CRBP1 gene was evident in liver (P < 0.01), but not in pituitary (P > 0.05). Overall, the expression level of CRBP1 gene in kidney, ovary and oviduct at the different ages had a "decline-rise-decline" trend in all three breeds. In contrast, in pituitary, hypothalamus, liver and kidney CRBP3 mRNA expression levels were significantly different at various ages (P < 0.05) and exhibited a "rise-decline-rise" pattern in all three breeds. Our results show that the expression of CRBP1 and CRBP3 in chicken tissues exhibit specific developmental changes and age-related patterns.
    Molecular Biology Reports 04/2014; 41(8). DOI:10.1007/s11033-014-3369-1 · 1.96 Impact Factor