The Unfolded Protein Response: Integrating Stress Signals Through the Stress Sensor IRE1

University of Santiago, Chile, CiudadSantiago, Santiago Metropolitan, Chile
Physiological Reviews (Impact Factor: 27.32). 10/2011; 91(4):1219-43. DOI: 10.1152/physrev.00001.2011
Source: PubMed


Stress induced by accumulation of unfolded proteins at the endoplasmic reticulum (ER) is a classic feature of secretory cells and is observed in many tissues in human diseases including cancer, diabetes, obesity, and neurodegeneration. Cellular adaptation to ER stress is achieved by the activation of the unfolded protein response (UPR), an integrated signal transduction pathway that transmits information about the protein folding status at the ER to the nucleus and cytosol to restore ER homeostasis. Inositol-requiring transmembrane kinase/endonuclease-1 (IRE1α), the most conserved UPR stress sensor, functions as an endoribonuclease that processes the mRNA of the transcription factor X-box binding protein-1 (XBP1). IRE1α signaling is a highly regulated process, controlled by the formation of a dynamic scaffold onto which many regulatory components assemble, here referred to as the UPRosome. Here we provide an overview of the signaling and regulatory mechanisms underlying IRE1α function and discuss the emerging role of the UPR in adaptation to protein folding stress in specialized secretory cells and in pathological conditions associated with alterations in ER homeostasis.

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    • "The liberated N-terminal cytoplasmic domain is transported into nuclei to activate UPR target genes. Inositol-requiring enzyme 1 is a type I ER-resident transmembrane protein with an ER luminal dimerization and a cytoplasmic domain with Ser/Thr kinase and endonuclease activities (Hetz et al., 2011). ER stress allows IRE1 to autophosphorylate the kinase domain through dimerization and thereby activate the ribonuclease domain. "
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    Plant Biotechnology Journal 06/2015; DOI:10.1111/pbi.12423 · 5.75 Impact Factor
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    • "IRE1/XBP-1 has been shown to regulate a variety of genes in various cell types in response to ER stress, mostly related to ER function and the secretory pathway, although the target genes vary depending on the cell type and nature of the stress stimuli [31]. In the proinsulin C96Y-GFP model of ER stress numerous genes related to ER function, the secretory pathway and ER-associated degradation are increased. "
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    ABSTRACT: Background The Akita mutation (C96Y) in the insulin gene results in early onset diabetes in both humans and mice. Expression of mutant proinsulin (C96Y) causes endoplasmic reticulum (ER) stress in pancreatic β-cells and consequently the cell activates the unfolded protein response (UPR). Since the proinsulin is terminally misfolded ER stress is irremediable and chronic activation of the UPR eventually activates apoptosis in some cells. Here we analyzed the IRE1-dependent activation of genes in response to misfolded proinsulin production in an inducible mutant proinsulin (C96Y) insulinoma cell line. Results The IRE1 endoribonuclease inhibitors 4μ8c and MKC-3946 prevented the splicing of the XBP1 mRNA in response to ER stress caused by mutant proinsulin production. Microarray expression analysis and qPCR validation of select genes revealed that maximal upregulation of many UPR genes in response to mutant proinsulin production required IRE1, although most were still increased above control. Interestingly, neither degradation of misfolded proinsulin via ER-associated degradation (ERAD), nor apoptosis induced by prolonged misfolded proinsulin expression were affected by inhibiting IRE1. Conclusions Although maximal induction of most UPR genes requires IRE1, inhibition of IRE1 does not affect ERAD of misfolded proinsulin or predispose pancreatic β-cells expressing misfolded proinsulin to chronic ER stress-induced apoptosis.
    BMC Cell Biology 07/2014; 15(1):29. DOI:10.1186/1471-2121-15-29 · 2.34 Impact Factor
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    • "Although GW501516 prevented the rise in CHOP protein levels after palmitate treatment in human cardiac cells, it did not reduce apoptosis. The relatively short duration of palmitate treatment might account for the lack of apoptotic activation in human cardiac cells, since several authors have speculated that the transition between adaptive UPR and apoptosis depends, at least in part, on the duration of ER stress stimulation [8]. It is widely accepted that, when ER stress is limited, the UPR potentiates autophagy as a short-term strategy to protect cells. "
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