Article

The expression of neuroglobin in astrocytoma.

Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China.
Brain Tumor Pathology (impact factor: 1.19). 01/2012; 29(1):10-6. DOI:10.1007/s10014-011-0066-9 pp.10-6
Source: PubMed

ABSTRACT Neuroglobin (NGB) is a characterized heme globin that is widely expressed in the vertebrate central and peripheral nervous system as well as retina and endocrine tissues. However, to date, it is not determined whether functional NGB is expressed in cells of glia origin. In this study, we aimed to explore the detailed expression of NGB in a rat astrocytoma cell line (C6) and human astrocytoma cell line (U251) by reverse transcription-polymerase chain reaction, immunofluorescence, and Western blotting, and to detect the expression of NGB in human astrocytoma tissues by an immunohistochemical method. We found that NGB was present in a rat astrocytoma cell line (C6), human astrocytoma cell line (U251), and human astrocytoma tissues. The expression and potential roles of NGB in astrocytomas may provide insight into the mechanisms of tumor cells to adapt and survive in hypoxic microenvironments and also represent a novel therapeutic approach to astrocytomas.

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    Article: Neuroglobin, a Novel Target for Endogenous Neuroprotection against Stroke and Neurodegenerative Disorders.
    Zhanyang Yu, Ning Liu, Jianxiang Liu, Kevin Yang, Xiaoying Wang
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    ABSTRACT: Brain neurons and tissues respond to sublethal injury by activating endogenous protective pathways. Recently, following the failure of a large number of clinical trials for protective strategies against stroke that aim to inhibit a specific ischemia response pathway, endogenous neuroprotection has emerged as a more promising and hopeful strategy for development of therapeutics against stroke and neurodegenerative disorders. Neuroglobin (Ngb) is an oxygen-binding globin protein that is highly and specifically expressed in brain neurons. Accumulating evidence have clearly demonstrated that Ngb is an endogenous neuroprotective molecule against hypoxic/ischemic and oxidative stress-related insults in cultured neurons and animals, as well as neurodegenerative disorders such as Alzheimer's disease, thus any pharmacological strategy that can up-regulate endogenous Ngb expression may lead to novel therapeutics against these brain disorders. In this review, we summarize recent studies about the biological function, regulation of gene expression, and neuroprotective mechanisms of Ngb. Furthermore, strategies for identification of chemical compounds that can up-regulate endogenous Ngb expression for neuroprotection against stroke and neurodegenerative disorders are discussed.
    International Journal of Molecular Sciences 01/2012; 13(6):6995-7014. · 2.60 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

characterized heme globin
 
detailed expression
 
endocrine tissues
 
functional NGB
 
glia origin
 
human astrocytoma cell line
 
human astrocytoma tissues
 
hypoxic microenvironments
 
immunohistochemical method
 
Neuroglobin
 
NGB
 
novel therapeutic approach
 
peripheral nervous system
 
potential roles
 
rat astrocytoma cell line
 
reverse transcription-polymerase chain reaction
 
vertebrate central
 
Western blotting