Development of a Highly Selective Fluorescence Probe for Hydrogen Sulfide

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Journal of the American Chemical Society (Impact Factor: 11.44). 11/2011; 133(45):18003-5. DOI: 10.1021/ja207851s
Source: PubMed

ABSTRACT Hydrogen sulfide (H(2)S) has recently been identified as a biological response modifier. Here, we report the design and synthesis of a novel fluorescence probe for H(2)S, HSip-1, utilizing azamacrocyclic copper(II) ion complex chemistry to control the fluorescence. HSip-1 showed high selectivity and high sensitivity for H(2)S, and its potential for biological applications was confirmed by employing it for fluorescence imaging of H(2)S in live cells.

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    • "We have utilized a variation of the lead acetate technique, optimized for tissue synthesis of H2S by methylene blue formation5. During 2011 a series of papers revealed new advances in applying highly selective fluorescence probes to measure H2S in living cells232425. We have combined the two techniques to investigate the regulation of leukocyte H2S synthesis. "
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    Scientific Reports 07/2012; 2:499. DOI:10.1038/srep00499 · 5.58 Impact Factor
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    ABSTRACT: Hydrogen sulfide (H2S) has emerged as an important biological signaling molecule. To better understand the multifaceted biological roles of H2S, the development of selective and sensitive biocompatible assays for H2S is becoming increasingly important. Motivated by these challenges, our laboratory is developing new methods to further detect and monitor biological H2S. Here, we describe in detail our recent advances in the development and the use of chemiluminescence-based H2S sensors to assist other investigators with use of these chemical tools. We highlight the use of these tools use by displaying their selectivity and high sensitivity toward H2S and provide examples of assays we have developed to detect enzymatically produced H2S. © 2015 Elsevier Inc. All rights reserved.
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    ABSTRACT: Since being identified in mammalian brain, hydrogen sulfide (H2S), a well-known toxic gas with a smell of rotten eggs, was predicted to have physiological functions. Three H2S-producing enzymes have since been identified and their physiological regulation has been intensively studied. The effects of H2S on neuromodulation, smooth muscle relaxation, inflammation, and angiogenesis indicate that it functions as a signaling molecule. It also protects the nervous system and the cardiovascular system from oxidative insults, suggesting a cytoprotective role for the molecule. In contrast to these physiological functions, pathophysiological roles for H2S have also been demonstrated. Patients with ethylmalonic encephalopathy have mutations in mitochondrial dioxygenase, causing high levels of H2S to be produced, with consequent damage to the brain and skeletal muscle. Levels of cystathionine β-synthase (CBS), an H2S-producing enzyme, are 3 times higher in patients with Down’s syndrome compared to normal individuals. In this article, in addition to the basic properties of H2S, physiological and pathophysiological aspects of this important molecule are reviewed.
    Gasotransmitters: Physiology and Pathophysiology, 01/2012: pages 71-98; , ISBN: 978-3-642-30337-1
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