Article

Immunomodulatory effect of human umbilical cord Wharton's jelly-derived mesenchymal stem cells on lymphocytes.

Bioengineering Department of Zhengzhou University, Zhengzhou, Henan Province, China.
Cellular Immunology (impact factor: 1.97). 01/2011; 272(1):33-8. DOI:10.1016/j.cellimm.2011.09.010 pp.33-8
Source: PubMed

ABSTRACT Studies have shown that mesenchymal stem cells (MSCs) have low immunogenicity and immune regulation. Human umbilical cord Wharton's jelly provides a new source for MSCs that are highly proliferative and have multi-differentiation potential. To investigate immunomodulatory effects of human Wharton's jelly cells (WJCs) on lymphocytes, we successfully isolated MSCs from human umbilical cord Wharton's jelly. WJCs expressed MSC markers but low levels of human leukocyte antigen (HLA)-ABC and no HLA-DR. These results indicate that WJCs have low immunogenicity. Both WJCs and their culture supernatant could inhibit the proliferation of phytohemagglutinin-stimulated human peripheral blood lymphocytes and mouse splenocytes. Additionally, WJCs suppressed secretion of transforming growth factor-β1 and interferon-γ by human peripheral blood lymphocytes. We conclude that the immunomodulatory effect of WJCs may be related to direct cell contact and inhibition of cytokine secretion by human peripheral blood lymphocytes.

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Keywords

culture supernatant
 
direct cell contact
 
growth factor-β1
 
human leukocyte antigen
 
human peripheral blood lymphocytes
 
human umbilical cord Wharton's jelly
 
human Wharton's jelly cells
 
immune regulation
 
immunomodulatory effect
 
immunomodulatory effects
 
low levels
 
MSC markers
 
multi-differentiation potential
 
new source
 
phytohemagglutinin-stimulated human peripheral blood lymphocytes
 
WJCs
 
WJCs suppressed secretion