Article

Towards multiple readout application of plasmonic arrays.

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany.
Beilstein Journal of Nanotechnology (impact factor: 0.79). 01/2011; 2:501-8. DOI:10.3762/bjnano.2.54 pp.501-8
Source: PubMed

ABSTRACT In order to combine the advantages of fluorescence and surface-enhanced Raman spectroscopy (SERS) on the same chip platform, a nanostructured gold surface with a unique design, allowing both the sensitive detection of fluorescence light together with the specific Raman fingerprint of the fluorescent molecules, was established. This task requires the fabrication of plasmonic arrays that permit the binding of molecules of interest at different distances from the metallic surface. The most efficient SERS enhancement is achieved for molecules directly adsorbed on the metallic surface due to the strong field enhancement, but where, however, the fluorescence is quenched most efficiently. Furthermore, the fluorescence can be enhanced efficiently by careful adjustment of the optical behavior of the plasmonic arrays. In this article, the simultaneous application of SERS and fluorescence, through the use of various gold nanostructured arrays, is demonstrated by the realization of a DNA detection scheme. The results shown open the way to more flexible use of plasmonic arrays in bioanalytics.

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Keywords

advantages
 
bioanalytics
 
careful adjustment
 
different distances
 
DNA detection scheme
 
efficient SERS enhancement
 
fabrication
 
flexible use
 
fluorescent molecules
 
metallic surface
 
nanostructured gold surface
 
open
 
optical behavior
 
plasmonic arrays
 
SERS
 
specific Raman fingerprint
 
surface-enhanced Raman spectroscopy
 
unique design
 
various gold nanostructured arrays