Development of an optimized backbone of FRET biosensors for kinases and GTPases. Mol Biol Cell

Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
Molecular biology of the cell (Impact Factor: 4.47). 12/2011; 22(23):4647-56. DOI: 10.1091/mbc.E11-01-0072
Source: PubMed


Biosensors based on the principle of Förster (or fluorescence) resonance energy transfer (FRET) have shed new light on the spatiotemporal dynamics of signaling molecules. Among them, intramolecular FRET biosensors have been increasingly used due to their high sensitivity and user-friendliness. Time-consuming optimizations by trial and error, however, obstructed the development of intramolecular FRET biosensors. Here we report an optimized backbone for rapid development of highly sensitive intramolecular FRET biosensors. The key concept is to exclude the "orientation-dependent" FRET and to render the biosensors completely "distance-dependent" with a long, flexible linker. We optimized a pair of fluorescent proteins for distance-dependent biosensors, and then developed a long, flexible linker ranging from 116 to 244 amino acids in length, which reduced the basal FRET signal and thereby increased the gain of the FRET biosensors. Computational simulations provided insight into the mechanisms by which this optimized system was the rational strategy for intramolecular FRET biosensors. With this backbone system, we improved previously reported FRET biosensors of PKA, ERK, JNK, EGFR/Abl, Ras, and Rac1. Furthermore, this backbone enabled us to develop novel FRET biosensors for several kinases of RSK, S6K, Akt, and PKC and to perform quantitative evaluation of kinase inhibitors in living cells.

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Available from: Kazuhiro Aoki, Mar 14, 2014
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    • "These substrate proteins include mediators of immediate changes in cell shape, movement and intermediary metabolism, and components of longer-term effects on gene expression, cell viability, division or differentiation (Hay, 2011; Manning and Cantley, 2007; Toker, 2012). A variety of FRET-based reporters have been developed to track Akt by live-cell imaging (Gao and Zhang, 2008; Komatsu et al., 2011; Kunkel et al., 2005; Miura et al., 2014; Yoshizaki et al., 2007). Collectively, they have yielded data demonstrating rapid induction of enzymatic function in response to signaling by different growth factors, but have provided little information about how Akt activity is encoded into signaling outputs or about the dynamics of responses within a cell population. "
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    Journal of Cell Science 06/2015; 128(14). DOI:10.1242/jcs.168773 · 5.43 Impact Factor
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    • "The basic modular design described above has been used to create activity reporters for a number of protein kinases, including PKA, protein kinase C (PKC), ataxia telangiectasia mutated (ATM), Akt, Abl, Src, aurora kinase B, ERK, c-Jun N-terminal kinase (JNK), cyclin-dependent kinase 1 (CDK1), AMP-activated protein kinase (AMPK), and the epidermal growth factor receptor (EGFR) (Zhang and Allen, 2007; Newman et al., 2011; Tsou et al., 2011; Belal et al., 2014). Using these reporters, researchers have uncovered important details about both the kinetics and the spatial distribution of endogenous kinase action in a variety of cellular contexts (Zhang et al., 2005a; Zhang and Allen, 2007; Erickson et al., 2011; Gao et al., 2011; Komatsu et al., 2011; Mehta and Zhang, 2011; Newman et al., 2011; Tsou et al., 2011; Arencibia et al., 2013; Ritt et al., 2013; Belal et al., 2014). However, despite their unique ability to track kinase activity in real time and at single cell resolution, to date, kinase activity reporters are available for <3% of the 518 human kinases in the human kinome. "
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    Frontiers in Genetics 08/2014; 5:263. DOI:10.3389/fgene.2014.00263
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    • "Coexpression of Bcr, but not a GAP-dead Bcr mutant (Bcr-GD), inhibited both Tiam1-induced Rac1 activation (Figure S1D) and autophosphorylation of Pak (Figures 1F and 1G), a Rac1 effector that regulates spine morphogenesis (Nikoli c, 2008). To measure the effect of Bcr on Tiam1-mediated Rac1 signaling in neurons, we utilized the Fö rster resonance energy transfer (FRET) Rac1 activation biosensor RaichuEV-Rac1 (Komatsu et al., 2011). Bcr overexpression decreased Rac1 activation in dendrites and spines both basally and when coexpressed with Tiam1 Figure 1. "
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