Association between MTHFR C677T genotype and circulating folate levels irrespective of folate intake: data from the IMMIDIET Project.

Research Laboratories, Fondazione di Ricerca e Cura "Giovanni Paolo II", Catholic University, Campobasso, Italy and National Reference Centre for Familial Hyperlipoproteinemias and Faculty of Nursing and Health Professional Studies, Slovak Medical University, Bratislava, Slovakia.
Nutrition (Impact Factor: 3.05). 11/2011; 27(11-12):1209-10. DOI: 10.1016/j.nut.2011.07.008
Source: PubMed
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    ABSTRACT: Objective Folate status has been associated with neural tube defects and cerebrovascular disease. We aimed at evaluating possible differences in folate status in two EU Countries and to assess their possible association with dietary patterns and/or other lifestyles. Methods and Procedures In the framework of the EU-funded IMMIDIET Project, 1,068 subjects (534 man-woman pairs), aged 26-64 years, were enrolled in Italy and UK. 1-year-recall Food Frequency Questionnaire was used to evaluate dietary intake. Reduced Rank Regression-analysis was used to derive a dietary pattern better describing high dietary folate intake. Results 11.3% of the Italians and 45.1% of the British exceeded the optimal dietary folate intake of 400μg/day (Recommended Dietary Allowance). 66.7% and 22.1% of Italian and UK women, respectively, all at childbearing age, had folate serum levels lower than 6.62ng/ml (P=0.01). The percentage of total variance of dietary folate intake explained by food group consumption was 14.2% and 16.3% in Italy and UK, respectively. Reduced Rank Regression-analysis indicated a healthy pattern which was positively associated with folate serum levels in both Countries (for all β-coef>0, P<0.001):100μg/day increase in dietary folate intake was associated with 13.8% and 10.5% increase in folate serum levels in the Italian and English population, respectively (for 100μg/day increase eβ-coef=1.138 and 1.105, P<0.001). Smoking habit was negatively but physical activity positively associated with folate serum levels (P<0.05). Conclusion(s) An inadequate dietary folate intake and subsequent serum levels were observed in Italian subjects. High consumption of food sources of folate was positively associated with folate serum levels, explaining a good proportion of its variability.
    Nutrition 01/2013; · 3.05 Impact Factor
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    ABSTRACT: Two genetic polymorphisms of methylenetetrahydrofolate reductase (MTHFR) gene (C677T and A1298C) can influence the plasma homocysteine (Hcy) levels, especially in the presence of an inadequate folate status. The aim of this study was to evaluate the frequencies of C677T and of A1298C MTHFR polymorphisms and their correlation with Hcy and serum folate concentrations in a population of blood donors living in a region of middle-southern Italy (the Molise Region). One hundred ninety seven blood donors were studied for total plasma Hcy, serum folate and C677T and A1298C MTHFR genotypes. The frequency of C677T genotypes was 20.8% (CC), 49.8% (CT) and 29.4% (TT); for the A1298C genotypes: 48.7% (AA), 43.7% (AC) and 7.6% (CC). Hcy and serum folate concentrations were significantly different among genotypes of the C677T polymorphism (CC versus CT versus TT: <0.0001 both for Hcy and folate), with Hcy values increasing, and serum folate decreasing, from CC to TT subjects. Regarding to A1298C polymorphism, the difference among genotypes (AA versus AC versus CC; p: 0.026 for Hcy and 0.014 for serum folate), showed an opposite trend for both parameters, with Hcy higher in the wild-type and lower in the homozygotes and serum folate higher in CC than in AA subjects. In conclusion, we found a high frequency of MTHFR allele associated with high level of Hcy and low levels of folate in an Italian southern population. Copyright © 2013 John Wiley & Sons, Ltd.
    Cell Biochemistry and Function 11/2013; · 2.13 Impact Factor