Article

Optical Coherence Tomography for live imaging of mammalian development

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, United States.
Current opinion in genetics & development (Impact Factor: 8.57). 09/2011; 21(5):579-84. DOI: 10.1016/j.gde.2011.09.004
Source: PubMed

ABSTRACT Understanding the nature and mechanism of congenital defects of the different organ systems in humans has heavily relied on the analysis of the corresponding mutant phenotypes in rodent models. Optical Coherence Tomography (OCT) has recently emerged as a powerful tool to study early embryonic development. This non-invasive optical methodology does not require labeling and allows visualization of embryonic tissues with single cell resolution. Here, we will discuss how OCT can be applied for structural imaging of early mouse and rat embryos in static culture, cardiodynamic and blood flow analysis, and in utero embryonic imaging at later stages of gestation, demonstrating how OCT can be used to assess structural and functional birth defects in mammalian models.

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Available from: Mary E Dickinson, Mar 27, 2014
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    • "Optical coherence tomography (OCT) allows for noninvasive, fast, single-cell resolution imaging through several millimeters of biological tissue. OCT, with its ability to provide accurate information on structure and dynamics, has been used for studying whole mammalian and avian embryos [1], [2], understanding hemodynamics [3], [4], and capturing 3D+time cardiac images in mammalian and avian embryos [5]–[7]. Frame rates currently achievable with direct, 3D+time OCT are too low to properly capture the dynamics of fast moving cardiovascular structures such as the beating embryonic heart. "
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