Article

Track structure, radiation quality and initial radiobiological events: Considerations based on the PARTRAC code experience

Laboratorio Energia Nucleare Applicata, Università degli studi di Pavia, Pavia, Italy.
International Journal of Radiation Biology (Impact Factor: 1.84). 09/2011; 88(1-2):77-86. DOI: 10.3109/09553002.2011.627976
Source: PubMed

ABSTRACT The role of track structures for understanding the biological effects of radiation has been the subject of research activities for decades. The physics that describes such processes is the core Monte Carlo codes, such as the biophysical PARTRAC (PARticle TRACks) code described in this review, which follow the mechanisms of radiation-matter interaction from the early stage. In this paper a review of the track structure theory (and of its possible extension concerning non-DNA targets) is presented.
The role of radiation quality and track structure is analyzed starting from the heavy ions results obtained with the biophysical Monte Carlo code PARTRAC (PARticles TRACks). PARTRAC calculates DNA damage in human cells based on the superposition of simulated track structures in liquid water to an 'atom-by-atom' model of human DNA.
Calculations for DNA fragmentation compared with experimental data for different radiation qualities are illustrated. As an example, the strong dependence of the complexity of DNA damage on radiation track structure, and the very large production of very small DNA fragments (lower than 1 kbp (kilo base pairs) usually not detected experimentally) after high LET (high-Linear Energy Transfer) irradiation is shown. Furthermore the possible importance of non-nuclear/non-DNA targets is discussed in the particular case of cellular membrane and mitochondria.
The importance of the track structure is underlined, in particular the dependence of a given late cellular effect on the spatial distribution of DNA double-strand breaks (DSB) along the radiation track. These results show that the relative biological effectiveness (RBE) for DSB production can be significantly larger than 1. Moreover the cluster properties of high LET radiation may determine specific initial targets and damage evolution.

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    • "If valid, these fi ndings add to the story of the importance of understanding 2003b). Th is would be consistent with the densely ionizing structure of an α -particle track not only resulting in complex DNA double-strand breaks (a DSB with associated additional damage sites within a few base pairs) but also, a correlation of DSB sites across nucleosomes or chromatin fi bre resulting in small DNA fragments and potentially impacting on the effi ciency of repair (Lobrich et al. 1996, Alloni et al. 2012). "
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