Isolation, characterization, and cross-amplification of microsatellite markers for the Petunia integrifolia (Solanaceae) complex

Molecular Evolution Laboratory, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
American Journal of Botany (Impact Factor: 2.6). 09/2011; 98(10):e277-9. DOI: 10.3732/ajb.1100178
Source: PubMed


Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida.
We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex.
The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.

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Available from: Luciano B Beheregaray,
    • "This is consistent with the findings of previous studies of plastid sequences, cleaved amplified polymorphic sequence (CAPS) nuclear markers (Segatto et al. 2014a; Turchetto et al. 2014a), and the phylogenetic position of these taxa (Reck-Kortmann et al. 2014). The mean number of alleles per locus obtained using EST-SSRs in these species (ten for a complete data set) was higher than that obtained in Petunia integrifolia based on genomic SSRs (a mean of three alleles per locus; Kriedt et al. 2011). A recent study involving wild Petunia species and commercial P. hybrida (Segatto et al. 2014b) showed that P. integrifolia species complex share several alleles and also have a low mean number of alleles per locus (five in Petunia inflata, four in P. "
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