Isolation, characterization, and cross-amplification of microsatellite markers for the Petunia integrifolia (Solanaceae) complex.

Molecular Evolution Laboratory, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
American Journal of Botany (Impact Factor: 2.46). 09/2011; 98(10):e277-9. DOI: 10.3732/ajb.1100178
Source: PubMed

ABSTRACT Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida.
We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex.
The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.

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