Comparison of the INNO-LiPA and PapType Assays for Detection of Human Papillomavirus in Archival Vulva Dysplasia and/or Neoplasia Tissue Biopsy Specimens

Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Victoria 3052, Australia.
Journal of clinical microbiology (Impact Factor: 4.23). 09/2011; 49(11):3980-2. DOI: 10.1128/JCM.00516-11
Source: PubMed

ABSTRACT INNO-LiPA and PapType human papillomavirus (HPV) genotyping assays were compared for detection of HPV genotypes on archival vulvar tissue. The INNO-LiPA assay detected 49 HPV-16 infections, compared with 47 detected by the PapType assay. The INNO-LiPA assay detected amplifiable DNA in 59 (91%) biopsy specimens, compared with 57 (88%) specimens for which amplifiable DNA was detected by the PapType assay. The two genotyping assays were highly comparable.

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Available from: Sepehr Tabrizi, Jul 30, 2015
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    • "Cuzick). 1 Deceased. 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) in a single assay [7] [8], and assessed whether the identification of more HR HPV genotypes could improve the triage of HR HPV positive women. "
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    ABSTRACT: Background HR HPV genotypes when assayed collectively, achieve high sensitivity but low specificity for the prediction of CIN2+. Knowledge of the specific genotypes in an infection may facilitate the use of HR HPV detection in routine clinical practice. Objectives To compare the rate of HR HPV detection and the accuracy of CIN2+ prediction between PapType test (Genera Biosystems) and other commercially available HR HPV assays, and to examine the value of full HPV genotyping. Study design. PreservCyt samples from 1099 women referred for abnormal cervical cytology were used. CIN2+ was chosen as the primary end-point but CIN3+ was also evaluated. A hierarchy of HR HPV genotypes was created using PPV and this was used to create 3 groups of genotypes with potentially different management. Results The PapType assay has a specificity of 22.4% and a sensitivity of 94.6% for CIN2+ prediction. Classification into Groups A (HPV33 and HPV16, very highly predictive), B (HPV31, 18, 52, 35, 58, 51 highly predictive) and C (HPV68, 45, 39, 66, 56, 59, intermediate predictive) could double the specificity (44.5%) but only slightly reduce the sensitivity for CIN2+ (91.5%) and CIN3+ (94.0%). Conclusions The PapType assay is a simple, reproducible and effective test for HR HPV detection and genotyping. HPV 33 was found to have a very high PPV and should therefore be managed as for HPV16.
    Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 05/2014; 60(1). DOI:10.1016/j.jcv.2014.02.002 · 3.47 Impact Factor
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    • "For paraffin-embedded archival samples, two commercially available assays utilizing PCR and targeting 65 and 150 bp region of L1 were used. The smaller amplicons are particularly suited for archival tissue [25] [26] [27]. 5–10 mm sections of FFPE archival tissue were deparaffinised with 800 í µí¼‡L histolene then mixed with 400 í µí¼‡L absolute ethanol. "
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