The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia.

Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, BPW, 1173 Sfax, University of Sfax, Tunisia.
Lipids in Health and Disease (Impact Factor: 2.31). 09/2011; 10:161. DOI: 10.1186/1476-511X-10-161
Source: PubMed

ABSTRACT In order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of M. vulgare L. essential oil against different pathogenic microorganisms and the cytotoxic activity against HeLa cell lines.
The agar disk diffusion method was used to study the antibacterial activity of M. vulgare essential oil against 12 bacterial and 4 fungi strains. The disc diameters of zone of inhibition (DD), the minimum inhibitory concentrations (MIC) and the concentration inhibiting 50% (IC50) were investigated to characterize the antimicrobial activities of this essential oil. The in vitro cytotoxicity of M. vulgare essential oil was examined using a modified MTT assay; the viability and the IC50 were used to evaluate this test.
The antimicrobial activity of the essential oil was investigated in order to evaluate its efficacy against the different tested microorganisms. The present results results showed a significant activity against microorganisms especially Gram (+) bacteria with inhibition zones and minimal inhibitory concentration values in the range of 6.6-25.2 mm and 1120-2600 μg/ml, respectively, whereas Gram (-) bacteria exhibited a higher resistance. As far as the antifungal activity, among four strains tested, Botrytis cinerea exhibited the strongest activity with inhibition zones of 12.6 mm. However, Fusarium solani, Penicillium digitatum and Aspergillus niger were less sensitive to M. vulgare essential oil. About the citotoxicity assay, this finding indicate the capability of this essential oil to inhibited the proliferation of HeLa cell lines under some conditions with IC50 value of 0.258 μg/ml.
This investigation showed that the M. vulgare essential oil has a potent antimicrobial activity against some Gram (+) pathogenic bacteria and Botrytis cinerea fungi. The present studies confirm the use of this essential oil as anticancer agent. Further research is required to evaluate the practical values of therapeutic applications.

1 Bookmark
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Many food preservation strategies can be used for the control of microbial spoilage and oxidation; however, these quality problems are not yet controlled adequately. Although synthetic antimicrobial and antioxidant agents are approved in many countries, the use of natural safe and effective preservatives is a demand of food consumers and producers. This paper proposes medicinal plants, traditionally used to treat health disorders and prevent diseases, as a source of bioactive compounds having food additive properties. Medicinal plants are rich in terpenes and phenolic compounds that present antimicrobial and antioxidant properties; in addition, the literature revealed that these bioactive compounds extracted from other plants have been effective in food systems. In this context, the present hypothesis paper states that bioactive molecules extracted from medicinal plants can be used as antimicrobial and antioxidant additives in the food industry.
    Journal of Food Science 01/2014; · 1.78 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In vitro antioxidant activity, total phenolic content and concentration of flavonoids of Marrubium vulgare methanolic extract were determined using spectrophotometric methods. The antibacterial activity was also evaluated against seven pathogenic bacteria using the disc-diffusion assay and broth dilution method. The obtained results showed that the tested extract was rich in phenol (26.8±0.01 mg gallic acid per gram) than in flavonoids (0.61 ±0.05 mg catechin equivalents per ml). The condensed tannin content was 0.086±0.01 mg ER/mL. In addition, the methanolic extract showed a strong antioxidant power against the DPPH (IC50= 35±0.01) and ABTS (IC50= 25±0.2) radicals scavenging. The tested extract had significant activity against both Gram-positive (B. cereus, L. monocytogens, S. aureus) and Gram-negative bacteria (E. coli, P. aeruginosa, A. hydrophila, S. typhimurium). The inhibition zones diameters and minimal inhibitory concentration values were in the range of 13-17 mm and 12.5-25 mg/ml, respectively. The high contents of phenolic compounds indicated that these compounds contribute to the antioxidant and antibacterial activities. The M. vulgare methanolic extract can be regarded as promising candidate for natural plant source of antioxidant and antimicrobial.
    Tunisian Journal of Medicinal Plants and Natural Products (TJMPNP). 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Introduction Not surprisingly, the upward desire in capturing the wisdom of traditional healing systems in management of different sorts of diseases among the nations, has led to a renewal of interest in herbal medicines. In this regard, plants with a wide range of biologically active constituents, at all times, have been providing scientists with innovative visions both in their natural forms and also by templates for novel molecular prototypes of drugs. Followed by a variety of investigations on a fundamentally unregulated cell growth, cancer has remained a major health problem worldwide with a choice of different expressions and pathologies. Seeing as the deadly nature of cancer, there exists severe scientific challenge among the researchers to understand the disease processes headed for discovery of potential fresh therapies from natural products. It has been accepted that an imbalance between the production of oxidants and frequency of antioxidant defenses namely oxidative stress could be one of the ensuing factors in DNA and protein damage, cancer, ageing, lipid peroxidation and inflammatory activities. 1 Following free radicals that are generated during oxidative stress with unpaired electrons seek for stability through electron pairing with biological macromolecules like proteins, lipids, and DNA of healthy human cells, bringing in about serious consequences of the oxidative stress conditions. In this issue, breast cancer developing from the metastatic progress of primary stage of cell tumors, has been considered as the prevalent malignancy among women the foremost cause of cancer related death. 2 Even so, phyto medicines confirming to be appealing sources of new compounds with new applications in clinical stages seem to have much to offer in treatment of cancers bringing about rational opportunities in this filed. 3-5
    Advanced pharmaceutical bulletin. 10/2014; 4(Suppl 1):459-464.

Full-text (2 Sources)

Available from
Jun 4, 2014