Article

Lectin microarrays: A powerful tool for glycan-based biomarker discovery

Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
Combinatorial chemistry & high throughput screening (Impact Factor: 1.93). 09/2011; 14(8):711-9. DOI: 10.2174/138620711796504398
Source: PubMed

ABSTRACT Cell surfaces, especially mammalian cell surfaces, are heavily coated with complex poly- and oligosaccharides, and these glycans have been implicated in many functions, such as cell-to-cell communication, host-pathogen interactions and cell matrix interactions. Not surprisingly then, the aberrations of glycosylation are usually indicative of the onset of specific diseases, such as cancer. Therefore, glycans are expected to serve as important biomarkers for disease diagnosis and/or prognosis. Recent development of the lectin microarray technology has allowed researchers to profile the glycans in complex biological samples in a high throughput fashion. This relatively new tool is highly suitable for both live cell and cell lysate analyses and has the potential for rapid discovery of glycan-based biomarkers. In this review, we will focus on the basic concepts and the latest advances of lectin microarray technology. We will also emphasize the application of lectin microarrays for biomarker discovery, and then discuss the challenges faced by this technology and potential future directions. Based on the tremendous progress already achieved, it seems apparent that lectin microarrays will soon become an indispensible tool for glycosylation biomarker discovery.

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Available from: Li Cheng, Apr 22, 2015
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    • "To overcome the drawbacks of the traditional lectin based methodologies, combined with the powerful characteristics of microarray format: high-throughput, parallel analysis and miniaturized format, we and others have developed a variety types of lectin microarrays carrying a few to ~100 lectins [17-21]. These lectin microarrays were fabricated on several different functionalized substrate surfaces, such as aldehyde, epbully and NHS derivatized hydrogel. "
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    • "Cell surface glycans are highly related to cell-cell communication, host-pathogen interaction, and cell matrix interactions [24]. Tumor invasion is dependent on a loss of intercellular adhesion and transmigration of cells through the basement membrane (BM) as well as the surrounding extracellular matrix (ECM) [25]. "
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