Erickson, B., Nelson, J.E. & Winters, P. Perspective on opportunities in industrial biotechnology in renewable chemicals. Biotechnol. J. 7(2), 176–185

Biotechnology Industry Organization (BIO), Washington, DC, USA.
Biotechnology Journal (Impact Factor: 3.49). 02/2012; 7(2):176-85. DOI: 10.1002/biot.201100069
Source: PubMed


From biomass to renewable chemicals: while industrial biotechnology offers a clear value proposition, a number of hurdles need to be addressed to fully realize the commercial potential of bio-based products and chemicals over the coming decade. A review of an early roadmap for biological production of chemicals from renewable sugars reveals a focus on those that would provide co-products for integrated biorefineries producing biofuels and bioenergy. A growing number of companies are now focusing on specialty chemicals as an entry point to build the bio-based economy.

Download full-text


Available from: Paul Winters, Oct 04, 2015
59 Reads
  • Source
    • "The genomic and metabolic diversity of prokaryotic domains of life is an extraordinary source for the development of innovative bio-based products of a high application value (Erickson et al. 2012; Buschke et al. 2013; Cragg and Newman 2013; He et al. 2014; Yu 2014). Marine environments contain an exceptional biodiversity generated and supported by a diversified range of special substrates and extreme conditions, such as high and low temperatures, extreme pH values, elevated salinities, pressure, and even irradiation. "
    [Show abstract] [Hide abstract]
    ABSTRACT: A metagenomic fosmid expression library established from environmental DNA (eDNA) from the shallow hot vent sediment sample collected from the Levante Bay, Vulcano Island (Aeolian archipelago) was established in Escherichia coli. Using activity-based screening assays, we have assessed 9600 fosmid clones corresponding to approximately 350 Mbp of the cloned eDNA, for the lipases/esterases/lactamases, haloalkane and haloacid dehalogenases, and glycoside hydrolases. Thirty-four positive fosmid clones were selected from the total of 120 positive hits and sequenced to yield ca. 1360 kbp of high-quality assemblies. Fosmid inserts were attributed to the members of ten bacterial phyla, including Proteobacteria, Bacteroidetes, Acidobateria, Firmicutes, Verrucomicrobia, Chloroflexi, Spirochaetes, Thermotogae, Armatimonadetes, and Planctomycetes. Of ca. 200 proteins with high biotechnological potential identified therein, we have characterized in detail three distinct α/β-hydrolases (LIPESV12_9, LIPESV12_24, LIPESV12_26) and one new α-arabinopyranosidase (GLV12_5). All LIPESV12 enzymes revealed distinct substrate specificities tested against 43 structurally diverse esters and 4 p-nitrophenol carboxyl esters. Of 16 different glycosides tested, the GLV12_5 hydrolysed only p-nitrophenol-α-(L)-arabinopyranose with a high specific activity of about 2.7 kU/mg protein. Most of the α/β-hydrolases were thermophilic and revealed a high tolerance to, and high activities in the presence of, numerous heavy metal ions. Among them, the LIPESV12_24 was the best temperature-adapted, retaining its activity after 40 min of incubation at 90 °C. Furthermore, enzymes were active in organic solvents (e.g., >30 % methanol). Both LIPESV12_24 and LIPESV12_26 had the GXSXG pentapeptides and the catalytic triads Ser-Asp-His typical to the representatives of carboxylesterases of EC
    Applied Microbiology and Biotechnology 08/2015; DOI:10.1007/s00253-015-6873-x · 3.34 Impact Factor
    • "The market share of succinic acid is expected to grow sevenfold by 2020 [35]. On subsequent hydrogenation, 1,4-butanediol is synthesized, which alone has a potential market of about US$3000 million [36]. Other important derivatives of succinic acid are tetrahydrofuran and g-butyrolactone, which are used as cleaning solvents for electronics and pharmaceuticals. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Biomass as an alternative to fossil fuels has shown significant potential to produce fuels and chemicals. Analogous to a petrochemical refinery, a biorefinery can be envisaged, where a diverse variety of products will be synthesized. These products can be made either through biological or chemical catalytic routes. Technologies relying solely on biological or chemical conversion methods have shown limitations in achieving high yield of a desired product. As an alternative, an integrated processing strategy can be applied where an intermediate chemical (e.g. lactic acid) or platform molecule is produced through biological transformation, which can be upgraded into useful compounds (e.g. propylene glycol) by chemical catalysis. This study is focused on such integrated methods, which have been applied to produce commodity chemicals and fuels. For the development of an integrated process, heterogeneous catalysts are required, which may be deactivated by the interaction of biogenic impurities, present in the fermentation media. A detailed overview of the available knowledge is presented on the mechanism leading to the deactivation of the catalyst surface by biogenic impurities, which will aid in the better design of the catalyst and purification process. In this regard, electrodialytic purification of the fermentation media is explored as an option for providing economic extraction of the product suited for subsequent catalytic processing. While studies on biogenic impurities are limited only to the hydrogenation reaction on metal surfaces, similar understandings can be applied to other catalytic reactions. Fundamental concepts introduced in this chapter can thus be applied for the development of an integrated process for the conversion of biomass-derived novel platform molecules into high-value chemicals and fuels.
    Sustainable Catalytic Processes, Edited by Basudeb Saha, Maonhong fan, Jianji Wang, 06/2015: chapter Chapter 6: pages 157-177; Elsevier., ISBN: 978-0-444-59567-6
  • Source
    • "To minimize these issues they can be replaced by biodegradable and more biocompatible organic salts or polymers. These include polypropylene glycol (propylene glycol is a generally recognized as safe (GRAS) additive for food and medical-related products), polyethylene glycol , natural polysaccharides, among others [31]. On the other hand, we are also facing an era where a vast plethora of ILs are available, and apart from the commonly studied imidazolium-based fluids, many of them are toxic to organisms and poorly biodegradable, ILs derived from natural sources, such as amino acids, carbohydrates or cholinium-based were already synthesized [32]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Aqueous biphasic systems (ABS) composed of ionic liquids (ILs) are promising platforms for the extraction and purification of proteins. In this work, a series of alternative and biocompatible ABS composed of cholinium-based ILs and polypropylene glycol were investigated. The respective ternary phase diagrams, tie-lines, tie-line lengths and critical points were determined at 25°C. The extraction performance of these systems for commercial bovine serum albumin (BSA) was then evaluated. The stability of BSA at the IL-rich phase was ascertained by size exclusion high-performance liquid chromatography (SE-HPLC) and Fourier transform infrared (FT-IR) spectroscopy. Appropriate ILs lead to the complete extraction of BSA for the IL-rich phase, in a single-step, while maintaining the protein's native conformation. Furthermore, to evaluate the performance of these systems when applied to real matrices, the extraction of BSA from bovine serum was additionally carried out, revealing that the complete extraction of BSA was maintained and achieved in a single-step. The remarkable extraction efficiencies obtained are far superior to those observed with typical polymer-based ABS. Therefore, the proposed ABS may be envisaged as a more effective and biocompatible approach for the separation and puri?cation of other value-added proteins. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Biotechnology Journal 04/2015; 10(9). DOI:10.1002/biot.201500003 · 3.49 Impact Factor
Show more