Crystal Structure of a Monomeric Retroviral Protease Solved by Protein Folding Game Players

Department of Biochemistry, University of Washington, Seattle, Washington, USA.
Nature Structural & Molecular Biology (Impact Factor: 13.31). 09/2011; 18(10):1175-7. DOI: 10.1038/nsmb.2119
Source: PubMed


Following the failure of a wide range of attempts to solve the crystal structure of M-PMV retroviral protease by molecular replacement, we challenged players of the protein folding game Foldit to produce accurate models of the protein. Remarkably, Foldit players were able to generate models of sufficient quality for successful molecular replacement and subsequent structure determination. The refined structure provides new insights for the design of antiretroviral drugs.

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Available from: Helena Zábranská, Sep 09, 2014
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    • "Canfield et al. 2002; Nicholson et al. 2002; Turner and Richter 2011; Donnelly et al. 2014), although level of engagement and tasks vary and might include result interpretation and analyses (e.g. Cardamone et al. 2009; Conrad and Hilchey 2011; Khatib et al. 2011; Macknick and Enders 2012; Lee et al. 2014). Citizen science projects have increased in importance and scope over the last decade (Miller- Rushing et al. 2012; Sauermann and Franzoni 2015) as a cost-effective alternative for acquiring high-resolution data and information. "
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    • "Recent advancements in engineering and technology have created an opportunity for citizen science to have a significant impact on scientific research. We have seen examples of this impact across many research fields through the discovery of protein structures [12], the identification of galaxies [13], and the validation of land cover classes [2]. This last reference [2], referring to the Geo-Wiki project, is the most recent example of a crowdsourcing effort to assist in environmental monitoring. "
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    • "The highest scoring solutions are analysed by researchers and validated if applicable in real problems or not. Users in Foldit has already helped to decipher the crystal structure of the Mason-Pfizer monkey virus retroviral protease [19]. "
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