Suppression of NF-κB pathway by crocetin contributes to attenuation of lipopolysaccharide-induced acute lung injury in mice

Department of pharmacology, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing 210009, PR China.
European journal of pharmacology (Impact Factor: 2.68). 09/2011; 674(2-3):391-6. DOI: 10.1016/j.ejphar.2011.08.029
Source: PubMed

ABSTRACT Crocetin, a carotenoid compound, has been shown to reduce expression of inflammation and inhibit the production of reactive oxygen species. In the present study, the effect of crocetin on acute lung injury induced by lipopolysaccharide (LPS) was investigated in vivo. In the mouse model, pretreatment with crocetin at dosages of 50 and 100 mg/kg reduced the LPS-induced lung oedema and histological changes, increased LPS-impaired superoxide dismutase (SOD) activity, and decreased lung myeloperoxidase (MPO) activity. Furthermore, treatment with crocetin significantly attenuated LPS-induced mRNA and the protein expressions of interleukin-6 (IL-6), macrophage chemoattractant protein-1 (MCP-1), and tumour necrosis factor-α (TNF-α) in lung tissue. In addition, crocetin at different dosages reduced phospho-IκB expression and NF-κB activity in LPS-induced lung tissue alteration. These results indicate that crocetin can provide protection against LPS-induced acute lung injury in mice.

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    • "It has also been shown that SEE contains many potent alkaloids and saponins (Hosseinzadeh & Younesi, 2002; Table 1). Other studies have speculated that the anti-inflammatory activity of SEE might be attributed to its content of crocin (Hemshekhar et al., 2012; Nam et al., 2010), crocetin (Nam et al., 2010; Yang et al., 2012) and safranal (Boskabady, Tabatabaee, & Byrami, 2012), agents whose individual anti-inflammatory effects were documented in the cited studies. "
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