Article

Molecular detection of Anaplasma phagocytophilum in Ixodid ticks in Hebei Province, China.

Key Laboratory of Preventive Veterinary Medicine in Hebei Province, Hebei Normal University of Science and Technology, 360 Hebei Street, Qinhuangdao, China.
Vector borne and zoonotic diseases (Larchmont, N.Y.) (impact factor: 2.61). 09/2011; 11(10):1323-7. DOI:10.1089/vbz.2010.0253
Source: PubMed

ABSTRACT A total of 3696 Ixodid ticks, collected from Hebei Province, China, were examined by a nested polymerase chain reaction for the presence of Anaplasma phagocytophilum. Forty-three (15.4%) of 280 pools tested, including 39 (14.6%) of 267 Haemaphysalis longicornis and four (30.8%) of 13 Dermacentor nuttalli, were positive, but no significant difference was found between D. nuttalli and H. longicornis (p>0.05). Sequence and phylogenetic analyses of 16S rRNA gene indicated that A. phagocytophilum in China is genetically diverse. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Hebei Province, China, and the first documentation of Anaplasma infection in D. nuttalli.

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Keywords

13 Dermacentor nuttalli
 
267 Haemaphysalis longicornis
 
Anaplasma infection
 
D. nuttalli
 
first documentation
 
first evidence
 
H. longicornis
 
Hebei Province
 
nested polymerase chain reaction
 
phylogenetic analyses