Discovery and structural characterization of a small molecule 14-3-3 protein-protein interaction inhibitor.
ABSTRACT The 14-3-3 family of phosphoserine/threonine-recognition proteins engage multiple nodes in signaling networks that control diverse physiological and pathophysiological functions and have emerged as promising therapeutic targets for such diseases as cancer and neurodegenerative disorders. Thus, small molecule modulators of 14-3-3 are much needed agents for chemical biology investigations and therapeutic development. To analyze 14-3-3 function and modulate its activity, we conducted a chemical screen and identified 4-[(2Z)-2-[4-formyl-6-methyl-5-oxo-3-(phosphonatooxymethyl)pyridin-2-ylidene]hydrazinyl]benzoate as a 14-3-3 inhibitor, which we termed FOBISIN (FOurteen-three-three BInding Small molecule INhibitor) 101. FOBISIN101 effectively blocked the binding of 14-3-3 with Raf-1 and proline-rich AKT substrate, 40 kD(a) and neutralized the ability of 14-3-3 to activate exoenzyme S ADP-ribosyltransferase. To provide a mechanistic basis for 14-3-3 inhibition, the crystal structure of 14-3-3ζ in complex with FOBISIN101 was solved. Unexpectedly, the double bond linking the pyridoxal-phosphate and benzoate moieties was reduced by X-rays to create a covalent linkage of the pyridoxal-phosphate moiety to lysine 120 in the binding groove of 14-3-3, leading to persistent 14-3-3 inactivation. We suggest that FOBISIN101-like molecules could be developed as an entirely unique class of 14-3-3 inhibitors, which may serve as radiation-triggered therapeutic agents for the treatment of 14-3-3-mediated diseases, such as cancer.
Article: Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine.[show abstract] [hide abstract]
ABSTRACT: The highly conserved and ubiquitously expressed 14-3-3 family of proteins bind to a variety of proteins involved in signal transduction and cell cycle regulation. The nature and specificity of 14-3-3 binding is, however, not known. Here we show that 14-3-3 is a specific phosphoserine-binding protein. Using a panel of phosphorylated peptides based on Raf-1, we have defined the 14-3-3 binding motif and show that most of the known 14-3-3 binding proteins contain the motif. Peptides containing the motif could disrupt 14-3-3 complexes and inhibit maturation of Xenopus laevis oocytes. These results suggest that the interactions of 14-3-3 with signaling proteins are critical for the activation of signaling proteins. Our findings also suggest novel roles for serine/threonine phosphorylation in the assembly of protein-protein complexes.Cell 04/1996; 84(6):889-97. · 32.40 Impact Factor
Article: Crystal structure of the 14-3-3zeta:serotonin N-acetyltransferase complex. a role for scaffolding in enzyme regulation.[show abstract] [hide abstract]
ABSTRACT: Serotonin N-acetyltransferase (AANAT) controls the daily rhythm in melatonin synthesis. When isolated from tissue, AANAT copurifies with isoforms epsilon and zeta of 14-3-3. We have determined the structure of AANAT bound to 14-3-3zeta, an association that is phosphorylation dependent. AANAT is bound in the central channel of the 14-3-3zeta dimer, and is held in place by extensive interactions both with the amphipathic phosphopeptide binding groove of 14-3-3zeta and with other parts of the central channel. Thermodynamic and activity measurements, together with crystallographic analysis, indicate that binding of AANAT by 14-3-3zeta modulates AANAT's activity and affinity for its substrates by stabilizing a region of AANAT involved in substrate binding.Cell 05/2001; 105(2):257-67. · 32.40 Impact Factor
Article: The eukaryotic host factor that activates exoenzyme S of Pseudomonas aeruginosa is a member of the 14-3-3 protein family.[show abstract] [hide abstract]
ABSTRACT: Exoenzyme S (ExoS), which has been implicated as a virulence factor of Pseudomonas aeruginosa, catalyzes transfer of the ADP-ribose moiety of NAD+ to many eukaryotic cellular proteins. Its preferred substrates include Ras and several other 21- to 25-kDa GTP-binding proteins. ExoS absolutely requires a ubiquitous eukaryotic protein factor, termed FAS (factor activating ExoS), for enzymatic activity. Here we describe the cloning and expression of a gene encoding FAS from a bovine brain cDNA library and demonstrate that purified recombinant FAS produced in Escherichia coli activates ExoS in a defined cell-free system. The deduced amino acid sequence of FAS shows that the protein (245 residues, calculated molecular mass 27,743 Da) belongs to a highly conserved, widely distributed eukaryotic protein family, collectively designated as 14-3-3 proteins. Various functions have been reported for members of the 14-3-3 family, including phospholipase A2 activity and regulation of tyrosine hydroxylase, tryptophan hydroxylase, and, possibly, protein kinase C activities. Identification of FAS as a 14-3-3 protein establishes an additional function for this family of proteins--the activation of an exogenous ADP-ribosyltransferase. Elucidation of the precise role of FAS in activating ExoS will contribute to understanding the molecular mechanisms by which P. aeruginosa causes disease.Proceedings of the National Academy of Sciences 04/1993; 90(6):2320-4. · 9.68 Impact Factor
Discovery and structural characterization of a small
molecule 14-3-3 protein-protein interaction inhibitor
Jing Zhaoa,b,1, Yuhong Dua,c,1, John R. Hortond,1, Anup K. Upadhyayd,1, Bin Loua, Yan Baia, Xing Zhangd, Lupei Due,
Minyong Lie, Binghe Wange, Lixin Zhanga,f, Joseph T. Barbierig, Fadlo R. Khurih, Xiaodong Chengd,2, and Haian Fua,h,c,2
aDepartments of Pharmacology;
School of Medicine, Atlanta, GA 30322;
Road, Xi’an 710032, China;
Sciences, Beijing 100101, China; and
bDepartment of Biochemistry and Molecular Biology, the Fourth Military Medical University, 17 Changle West
eDepartment of Chemistry, Georgia State University, Atlanta, GA 30302;
gDepartment of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226
hHematology and Medical Oncology;
cEmory Chemical Biology Discovery Center, Emory University
fInstitute of Microbiology, Chinese Academy of
Edited by Tony Pawson, Samuel Lunenfeld Research Institute, Toronto, Canada, and approved July 27, 2011 (received for review January 1, 2011)
The 14-3-3 family of phosphoserine/threonine-recognition proteins
engage multiple nodes in signaling networks that control diverse
physiological and pathophysiological functions and have emerged
as promising therapeutic targets for such diseases as cancer and
neurodegenerative disorders. Thus, small molecule modulators of
14-3-3 are much needed agents for chemical biology investigations
and therapeutic development. To analyze 14-3-3 function and mod-
ulate its activity, we conducted a chemical screen and identified
din-2-ylidene]hydrazinyl]benzoate as a 14-3-3 inhibitor, which we
termed FOBISIN (FOurteen-three-three BInding Small molecule IN-
hibitor) 101. FOBISIN101 effectively blocked the binding of 14-3-3
with Raf-1 and proline-rich AKT substrate, 40 kDaand neutralized
the ability of 14-3-3 to activate exoenzyme S ADP-ribosyltransfer-
ase. To provide a mechanistic basis for 14-3-3 inhibition, the crystal
structure of 14-3-3ζ in complex with FOBISIN101 was solved.
Unexpectedly, the double bond linking the pyridoxal-phosphate
and benzoate moieties was reduced by X-rays to create a covalent
linkage of the pyridoxal-phosphate moiety to lysine 120 in the
binding groove of 14-3-3, leading to persistent 14-3-3 inactivation.
We suggest that FOBISIN101-like molecules could be developed as
an entirely unique class of 14-3-3 inhibitors, which may serve as
radiation-triggered therapeutic agents for the treatment of 14-3-
3-mediated diseases, such as cancer.
small molecule 14-3-3 modulator
τ, and ζ) in mammals and is widely expressed in all tissues and
organs examined (1–3). 14-3-3 acts as an adaptor protein that
controls the function of its target proteins through highly regu-
lated protein–protein interactions. Studies on the interaction of
14-3-3 with phosphorylated Raf-1 led to the discovery of 14-3-3 as
the founding member of the class of phosphoserine/threonine-
binding protein modules (4–8). Reversible phosphorylation of
target proteins at a defined motif dictates the 14-3-3 association
in response to dynamic actions of cellular kinases and phospha-
tases. These 14-3-3 recognition motifs include the prototype
COOH (mode 3), where x stands for any amino acid (8–11). The
availability of well characterized 14-3-3 recognition motifs coupled
with advanced genomics, proteomics, and functional biology ap-
proaches has revealed an entirely new landscape in which 14-3-3
binds a variety of signaling molecules, controlling their function
in response to environmental signals (8, 9, 12–14). More than 200
ligand proteins have been identified for 14-3-3 (12). Depending on
the nature of its target proteins, 14-3-3 binding impacts multiple
signaling pathways that determinecell fate and organ development.
For example, 14-3-3 association controls Raf signaling fidelity,
neutralizes Bad-mediated apoptosis, and couples histone H3 with
H4 to create a histonecode for transcriptional elongation (2, 3, 15).
Through these highlyregulated interactions, 14-3-3 proteins govern
nitially discovered as a protein abundant in the brain, the 14-3-3
family ofproteins consists of seven defined isoforms (β,ϵ,γ, η,σ,
diverse physiological processes as well as a wide range of pathophy-
siological events. For example, dysregulated 14-3-3 signaling con-
tributes to the development of a number of human diseases, such
as cancer and neurodegenerative diseases (1–3). Thus, 14-3-3 pro-
teins are promising molecular targets for probe discovery and
In an effort to discover 14-3-3 protein–protein interaction
modulators, we have previously reported the development and
structural characterization of peptide 14-3-3 antagonists, R18
and difopein (16–18), which have been widely used in the field
to manipulate 14-3-3/client protein interactions for functional
studies. It is expected that small molecule 14-3-3 modulator dis-
covery would provide added advantages to rapidly advance the
14-3-3 field, which impacts a wide range of biomedical areas.
Here we report our experimental chemical screening effort, the
identification and analysis of FOBISIN101 as a phosphoSer/Thr-
mimetic agent, and the structural details of the FOBISIN101∕
14-3-3ζ interaction. This study revealed an unexpected covalent
modification of 14-3-3ζ by a FOBISIN 101 derivative at a critical
ligand binding site, Lys120, explaining its potent 14-3-3 inhibitory
Results and Discussion
Using a fluorescence polarization-based 14-3-3 binding assay
(19), we screened the LOPAC library for compounds that disrupt
the interaction of 14-3-3γ with the pS259-Raf-1 peptide and
identified FOBISIN101 (F1 in Fig. 1A) as a potential 14-3-3 in-
hibitor (Fig. S1) (19, 20). F1 consists of a pyridoxal-phosphate
moiety linked to p-amino-benzoate via an N ¼ N bond. Because
the screening assay utilized a 14-3-3-binding peptide, it is essen-
tial to demonstrate that FOBISIN101 is capable of disrupting
the interaction of 14-3-3 with its full-length binding proteins.
We employed three complementary biochemical and functional
assays for this purpose. AGlutathione S-transferase (GST) fusion
14-3-3 affinity chromatography assay was used to examine the
ability of F1 to disrupt the 14-3-3 association with two well estab-
lished partners, Raf-1 and proline-rich AKT substrate, 40 kDa
(PRAS40). The addition of increasing concentrations of F1 to
the cell lysates led to a dose-dependent release of PRAS40 and
Raf-1, supporting an effective inhibitory role of F1. This inhibi-
Author contributions: Y.D., F.R.K., X.C., and H.F. designed research; J.Z., Y.D., J.R.H., A.K.U.,
B.L., Y.B., and X.Z. performed research; B.L., L.D., M.L., B.W., L.Z., and J.T.B. contributed
new reagents/analytic tools; J.Z., Y.D., J.R.H., A.K.U., Y.B., X.Z., L.Z., F.R.K., X.C., and H.F.
analyzed data; and J.Z., Y.D., J.R.H., A.K.U., X.C., and H.F. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
Data deposition: The atomic coordinates have been deposited in the Protein Data Bank,
www.pdb.org (PDB ID code 3RDH).
1J.Z., Y.D., J.R.H., A.K.U. contributed equally to this work.
2To whom correspondence may be addressed. E-mail: firstname.lastname@example.org or xcheng@
This article contains supporting information online at www.pnas.org/lookup/suppl/
16212–16216 ∣ PNAS ∣ September 27, 2011 ∣ vol. 108 ∣ no. 39www.pnas.org/cgi/doi/10.1073/pnas.1100012108
tion occurred in a manner similar to the action of a defined
14-3-3 antagonist peptide, R18 (17) (Fig. 1B). F1 appears to be
a pan-14-3-3 inhibitor as it decreased the binding of Raf-1 to all
seven 14-3-3 isoforms in a dose-dependent manner (Fig. S2). A
quantitative enzyme-linked immunosorbent assay established
a half-maximal inhibitory concentration (IC50) for F1, which was
9.3 or 16.4 μM, respectively, for the binding of 14-3-3ζ or 14-3-3γ
to PRAS40 (Fig. 1C). Furthermore, F1 effectively blocked the
ability of all seven 14-3-3 isoforms to stimulate exoenzyme S
(ExoS) ADP-ribosyltransferase (IC50¼ 6–19 μM) in a functional
assay (21) (Fig. 1D). Because ExoS is a nonphosphorylated client
protein, F1 is capable of interfering with the binding of both
phosphorylated and nonphosphorylated client proteins to 14-3-3.
Together, these data suggest a direct action of F1 on 14-3-3
To provide a structural explanation for the inhibitory effect
of F1 on 14-3-3 proteins, compound F1 was soaked into pre-
formed crystals of 14-3-3ζ. These cocrystals were bright orange
in color (Fig. 2A). During the X-ray data collection, immediately
after exposure to synchrotron radiation, the cocrystals turned
brownish, then yellow, and eventually became colorless. We de-
termined the complex structure to a resolution of 2.39 Å
(Table S1). The crystal contains four monomers (two dimers)
within the asymmetric unit (Fig. 2B), with a root-mean-square-
deviation of <0.5 Å of 230 pairs of Cα atoms between the mono-
mers. Each monomer consists of nine α helices that form an a
mphipathic groove where a client protein is located (9, 22–24).
F1 is bound to the basic surface of the peptide-binding groove
of each monomer. However, only the pyridoxal-phosphate moiety
of F1 was found in this groove (Fig. 2C). The F1 exocyclic nitro-
gen atom formed a covalent bond with the side chain terminal
nitrogen of Lys120, forming a diazene adduct with a N-N distance
of approximately 1.2 Å. The F1 phosphate group interacts with
the side chain of Lys49 and Asn173, while one face of the pyri-
doxal ring makes van der Walls contact with the side chain of
Ile217 (Fig. 2D, Fig. S3). These interactions position the F1 de-
rivative in a defined conformation. In addition, a solvent mole-
cule bridges Arg56 and Arg127.
We superimposed the F1-bound structure of 14-3-3ζ to that of
14-3-3 bound to either the pS259-Raf-1 (PDB 3CU8) or pS10-
histone H3 (25) (PDB 2C1N) peptide. In order to interact with
phosphorylated ligands, 14-3-3ζ engages a cluster of basic or
polar residues, including (i) Arg56, Arg127, and Tyr128, which
coordinates the binding of the phosphate group (Fig. 2E) and
(ii) Asn173, whose side chain oxygen atom forms a hydrogen
bond with the main chain amide nitrogen of the residue C-term-
inal to the phosphoserine and involves an intramolecular inter-
action network forming a hydrogen bond with Asp114, which
in turn forms a salt bridge with Lys120 (Fig. 2F). The conforma-
tion of peptide residues at þ2 and beyond differs when the pep-
tide exits from the 14-3-3 ligand binding groove, with Raf-1 to the
left and histone H3 to the right of Lys49, as shown in Fig. 2E.
It appears as though the phosphate group of covalently linked
F1 shifted approximately 4 Å away from the phosphoserine bind-
ing site, towards Lys120 (Fig. 2G). Mutagenesis of 14-3-3ζ
coupled with direct binding studies using isothermal titration
calorimetry indicated the importance of R56 and R60 in the bind-
ing of native uncleaved F1 (Fig. S4, Table S2), which supports
the proposed model in Fig. 2. We reasoned that the phosphate
moiety of F1 might be critical for its inhibitory activity by mimick-
ing the phosphorylated peptide motif for 14-3-3 binding. We thus
generated the compound F2, which lacks the phosphate group,
and observed that this compound had a drastically reduced effect
in blocking 14-3-3 binding to Raf-1 or PRAS40 (Fig. 1 B and C,
Fig. S2) and in inhibiting 14-3-3-mediated activation of ExoS
(Fig. 1E). Moreover, changes in the phenyl ring structure also
showed some effect on the 14-3-3/Raf-1 interaction (Fig. S5),
demonstrating the involvement of the azophenyl substructure
in 14-3-3 interaction. These data support F1 as a 14-3-3 inhibitor
and highlight its phosphate moiety as a primary functional com-
ponent. We modeled intact F1 by superimposing the F1 phos-
phate group onto that of phosphoserine and rotating the torsion
angles to reach maximum overlap with the bound peptide
(Fig. 3A). The model suggests that the side of the pyridoxal ring
with the phosphate group superimposes well with peptide back-
bones before and after phosphoserine, while the benzoate ring
14-3-3 to full-length Raf-1 and PRAS40 in cell lysates. GST-14-3-3γ was mixed with COS-7 cell lysate in the presence of test compounds. The 14-3-3γ complex was
isolated by GST-pull-down. The presence of Raf-1 and PRAS40 in the 14-3-3γ complex was revealed by Western blot analysis. (C) Inhibition of the interaction
between PRAS40 and 14-3-3γ or 14-3-3ζ by F1 in an ELISA assay. Interaction of PRAS40 with GST-14-3-3γ or 14-3-3ζ immobilized on an anti-GSTantibody-coated
plate gave rise to robust ELISA signals as detected by anti-PRAS40 antibody. (D) F1 inhibits ExoS activation by all seven isoforms of 14-3-3. ExoS and its substrates
were incubated with 14-3-3 proteins in the presence or absence of F1. 14-3-3-dependent ExoS enzymatic activity was quantified by the amount of32P-ADP-
ribose incorporated into SBTI, % control relative to samples without F1 was calculated. (E) Dephosphorylated F1 (F2) does not affect 14-3-3-mediated ExoS
activity in an ExoS assay as described in (D). The results in c − e were presented as means ? SD (n ¼ 3) of one representative experiment. The experiments were
repeated at least three times with similar results.
Discovery of FOBISIN (A) Chemical structures of FOBISIN 101 (F1), F2 (dephosphorylated F1), and F3 (pyridoxal-phosphate). (B) F1 blocks the binding of
Zhao et al.PNAS
September 27, 2011
could point to the peptide exit pathway similar to that of Raf-1
(Fig. 3A, left box).
To explore the possible cause of the covalent modification of
14-3-3 by F1, we hypothesized that radiation exposure cleaves the
N ¼ N diazene bond thereby releasing the paraaminobenzoic
acid moiety into the solvent, while the hydrogen binding inter-
action holds the pyridoxal-phosphate moiety in place within the
14-3-3 binding site (Fig. 3B). In this model, the reactive nitrogen
of the pyridoxal-phosphate group is approximately 6–7 Å from
either Lys120 or Lys49, respectively (Fig. 3C). However, the side
chain Nϵ of Lys120 is roughly parallel, while that of Lys49 is
roughly perpendicular, to the plane of the pyridoxal ring. We sug-
gest that bond-breaking and bond-making processes proceed
through specific attack trajectories. The preferred attack trajec-
tory might be the one which lies parallel to the plane of the ring
and facilitates the formation of a new nitrogen bond of the
cleaved compound with the side chain of Lys120, leading to cova-
lent modification and inactivation of 14-3-3 function. Indeed, mu-
tating Lys120 to Glu alone is sufficient to inactivate 14-3-3ζ
The N ¼ N bond in diazene compounds is generally sensitive
to radiation and is known to undergo photolysis to generate re-
active organic radicals (26). We note that another plausible
source of the covalent adduct is through imine (Schiff base) for-
mation between the ϵ-amino group of a lysine and the aldehyde
group (with the loss of water) on the pyridoxal-phosphate portion
of the inhibitor. However, three lines of evidence argue against
this possibility. First, the electron density is of sufficient quality
to identify the phosphate group, its associated pyridine ring, and
the aldehyde (with a two bond length -C ¼ O away from a ring
atom). If the site of covalent attachment is via imine formation,
irradiation-induced cleavage of the N ¼ N bond would be re-
duced to NH2(in this case, one bond length away from a ring
atom). Second, the covalent adduct is only formed after exposure
14-3-3ζ in the asymmetric unit. (C) Covalently modified Lys120 in the peptide-binding groove of 14-3-3ζ. Omit electron densities, Fo-Fc (red mesh) and 2Fo-Fc
(green mesh), contoured at 4σ and 1.2σ above the mean, respectively, are shown for F1-modified Lys120. (D) Details of 14-3-3ζ and F1 fragment interactions.
The letter w indicates a solvent [either water in molecule (A-C) or a partially occupied phosphate in molecule (D)]. (E) Superimposition of 14-3-3ζ in complex
with a Raf-1 peptide (blue) (PDB 3CU8) and a histone H3 peptide (gray) (PDB 2C1N). For clarity, H3 residues 12–14 (which point toward the viewer) were
removed. (F) Lys120 is involved in intramolecular interactions. (G) Superimposition of a covalently bound F1 fragment (yellow) with that of Raf-1 (blue)
and H3 (gray) phosphoserine peptides.
Structure of the 14-3-3ζ∕F1 fragment covalent complex (A) Example color change of the 14-3-3ζ∕F1 crystal after exposure to X-rays. (B) Two dimers of
idoxal-phosphate moiety and the phosphoserine of a Raf-1 peptide (left) or
histone H3 peptide (right). For clarity, H3 residues 13-14 (which point toward
the viewer) were removed. (B) Proposed bond-breaking and bond-making
process. (C) Lys120 lies in a position for a preferred attack trajectory. (D) Mass
spectrum profiles (in the reflection mode of acquisition of the spectrum)
showing a 14-3-3ζ fragment containing Lys120 digested from crystals with
(bottom) and without (top) exposure to X-rays. The addition of 182 Da cor-
responds to modification of Lys120 (262 Da) with the loss of a phosphate
group (HPO3) from the peptide (by a reduction of 80 Da in the mass), pre-
sumably due to laser (337 nm) induced metastable decomposition during the
MALDI ionization process (35–38).
Model of intact F1 bound to 14-3-3ζ (A) Superimposition of the pyr-
www.pnas.org/cgi/doi/10.1073/pnas.1100012108Zhao et al.
to X-rays. It is not known whether imine formation with pyridox-
al-phosphate requires photolysis. Gel digestion and mass spectro-
metric analysis of digested peptides obtained from F1-soaked
crystals that were not exposed to X-rays showed no adduct peak
(Fig. 3D, top box). Importantly, only the X-ray-treated samples
showed a mass addition corresponding to a peptide fragment of
residues 114-131 with modified Lys120 (Fig. 3D, bottom box).
Therefore, the fragmentation and covalent adduct formation
of F1 observed in the 14-3-3ζ crystal structure very well could
be induced by X-ray radiation during data collection, as evident
by the change of color (Fig. 2A). Third, assuming the site of cova-
lent attachment is via imine formation and the pyridine ring takes
on the same conformation (by flipping the ring 180° horizontally
as shown in Fig. 2C), the paraaminobenzoic acid moiety would
point to the solvent without any specific contact to the protein.
However, the pyridoxal-phosphate (F3 in Fig. 1A) has much re-
duced potency (Fig. 1E), indicating that the phenyl ring structure
still contributes to the inhibitory activity of F1, which is supported
by data in Fig. S5.
In conclusion, we have identified and experimentally con-
firmed a series of small molecule phospho-binding site inhibitor
and revealed structural details of one such molecule with 14-3-3ζ.
Because of the specificity of the mode of binding as revealed by
the cocrystal structural studies and the potent effect on both
phosphorylated and nonphosphorylated client protein binding
to 14-3-3 proteins, this pyridoxal-phosphate class of compounds
are expected to define a unique class of 14-3-3 inhibitors for phy-
siological and therapeutic investigations. It is important to note
that the F1 class of compounds have been investigated as iono-
tropic P2X receptor antagonists (20). Rich medicinal chemistry
information around this structural scaffold will greatly facilitate
their development as 14-3-3 modulators. Importantly, we also
offer a prodrug concept for 14-3-3-mediated diseases. For exam-
ple, F1-like molecules could be developed as radiation-triggered
therapeutic agents for the treatment of cancer. It is envisioned
that such 14-3-3 inhibitors alone may show negligible toxicity
to the host; however, radiation therapy targeted to a particular
tumor area may specifically cleave suchdesigned 14-3-3 inhibitors
and lead to their covalent modification and potent inactivation of
14-3-3 proteins in tumors.
Materials and Methods
Molecular and Cell Biology Reagents. The expression vectors for GST-14-3-3
and His-14-3-3 isoforms were constructed as previously described (27).
Glutathione agarose beads and nickel-affinity columns were purchased from
GE Healthcare. Anti-Raf-1 and anti-GSTantibodies were from Santa Cruz and
the anti-PRAS40 antibody was from Biosource. 14-3-3ζ was expressed in
Escherichia coli BL21(DE3) harboring pET-15b-derived plasmids and purified
using Ni2þchelating chromatography essentially as described (21). Hexahis-
tidine tags were removed by thrombin digestion. The 14-3-3 protein used
for crystallization was further purified by gel filtration chromatography
(Superdex 200 in a Pharmacia FPLC system). ExoS was purified as previously
described (28). The LOPAC library was purchased from Sigma-Aldrich. COS-7
cells were grown in DMEM supplemented with 10% FBS.
Florescence Polarization Assay and Chemical Screening. The 14-3-3 FP assay was
carried out in black 384-well microplates in a total volume of 50 μL (19). Assay
reaction buffer (49 μL: 1 μM GST-14-3-3γ and 2 nM TMR-pS259-Raf peptide in
Hepes buffer) was dispensed to each well. Test compound (1 μL of 2 mM stock
in DMSO) was added to the reaction buffer using a Sciclone liquid handler
(Caliper LifeSciences). Plates were incubated at room temperature and the FP
value in millipolarization (mP) units was recorded with an Analyst HT reader
(Molecular Devices). An excitation filter at 545 nm and an emission filter at
610 to 675 nm were used with a dichroic mirror at 565 nm. Data analysis
was conducted using CambridgeSoft software. Compounds with recorded
mP values less than three standard deviation from the negative controls were
considered positive hits.
Enzyme-Linked Immunosorbent Assay. The 14-3-3 ELISA assay was developed
in 96-well microplates coated with either anti-GST antibody or glutathione
(Pierce Biotechnology). This assay monitors the interaction of recombinant
GST-tagged 14-3-3 proteins with endogenous client proteins, such as PRAS40,
in COS-7 cell lysate. Briefly, GST-14-3-3 protein (1 μM) immobilized on an
anti-GST plate was incubated with a test compound before adding COS-7 cell
lysates in 1% NP-40 lysis buffer (16). After incubation and washing, antibo-
dies specific to PRAS40 along with peroxidase-labeled anti-rabbit IgG (50 μL;
1∶1;000 dilution) were added. After washing, 100 μL of tetramethylbenzidine
was added. The reaction was stopped with sulfuric acid (0.1 N) and recorded
at 450 nm on an Envision™ reader (Perkin Elmer). IC50values were calculated
using GraphPad software.
GST Pull-Down Assay and Western Blotting. For binding assays, GST-14-3-3 pro-
teins (1 μg) were preincubated with various concentrations of test compound
before COS-7 cell lysates were added. Client proteins associated with GST-14-
3-3 were captured by glutathione Sepharose beads while unbound proteins
were removed by washing (1% NP-40 buffer). The fraction that was bound
to the beads was analyzed by SDS-PAGE followed by immunoblotting with
antibodies specific to Raf-1, PRAS40, and GST.
ExoS Activation Assay. To examine the functional effect of test compounds on
14-3-3 proteins, we utilized the 14-3-3-dependent ExoS ADP-ribosyltransfer-
ase assay (21). This assay is used as a functional readout for 14-3-3 inhibitors.
Briefly, 14-3-3 protein was preincubated with test compounds, followed by
incubation with ExoS in the presence of substrates (SBTI, NAD, and 0.35 μCi of
½adenylate-32P?NADþas a reaction tracer). The reaction was terminated by
spotting assay mixture onto P81 phosphocellulose paper (Whatman). After
washing, radioactivity incorporated into SBTI by ExoS was determined by
liquid scintillation counting. Enzyme activities were expressed as picomoles
of ADP-ribose incorporated per min per microgram of ExoS. The inhibitory
effect of compounds was expressed as percent inhibition of ExoS activity over
Chemical Synthesis of Compound F2. A 5.1 mg sample of F1 was dissolved in
1.0 mL of hydrofluoric acid (48%, Sigma-Aldrich) and incubated in an ice bath
for 2 h. The solution was adjusted to a pH of 5 using a saturated NaOH aqu-
eous solution. The solution was dried under avacuum using arotary-evapora-
tor at 30°C. Precooled (−20°C) ethanol was added to the remaining residue.
The solid residue was filtered and the filtrate was dried to give 3.1 mg of a
brown solid product. Yield: 89%.
ESI-NEG: [M-H] 314.1
1H-NMR (400 MHz, CD3OD): 8.02–8.24 (d, 4H), 6.30 (m, 1H), 5.35–5.38
(m, 2H), 2.63 (s, 3H).
Crystallography. The 14-3-3ζ protein (10 mg∕mL in 20 mM Tris/HCl, pH 8.5,
100 mM NaCl) was crystallized by the hanging drop vapor diffusion method
using conditions similar to those previously described (22) (25% PEG3350,
100 mM Tris-HCl, pH 8.5, 10 mM MgCl2, 1 mM NiCl2, 1% glycerol at 16°C).
The 14-3-3ζ crystals were soaked with 5 mM F1 compound for 3 d. The crystals
were then momentarily immersed into well solution containing either 25%
ethylene glycol or glycerol as a cryoprotectorant and frozen by plunging into
liquid nitrogen. The crystals were then stored in a liquid nitrogen dewar until
data collection at the SET-CATsynchrotron beamline at the Advanced Photon
Source of Argonne National Laboratory. Structural determination of the
14-3-3ζ∕F1 complex proceeded by molecular replacement with the program
PHASER (29) using the 14-3-3 coordinates of a 14-3-3/ExoS complex (PDB
2O02) (30). Refinement of the model proceeded with manual manipulation
using the graphic programs COOT (31) and O (32) and computational manip-
ulation with the program CNS (33). On the basis of evident electron density,
we modeled the F1 modified Lys120, whose topology and parameter files for
refinement were obtained from the Dundee PRODRG2 Server (34) (http://
Mass Spectrometry. Covalent adduct formation between 14-3-3ζ-K120 and
fragmented F1 was verified by performing MALDI-TOF-MSanalysis of V8-pro-
tease (New England BioLab) digested peptide fragments of the F1-protein
crystals with or without X-ray exposure.
Detailed information onmass
calorimetry, mutagenesis analysis is included in the SI Materials and
Methods as well as additional information on FOBISIN 104–107.
ACKNOWLEDGMENTS. We thank Paul R. Thompson for critical comments. The
Department of Biochemistry at the Emory University School of Medicine sup-
ported the use of the SER-CATsynchrotron beamline at the Advanced Photon
Source of Argonne National Laboratory, local X-ray facility and MALDI-TOF
mass spectrometry and HTS was performed at the Emory Chemical Biology
Discovery Center. This work was supported in part by the National Institutes
Zhao et al.PNAS
September 27, 2011
of Health Grants P01 CA116676 (to F.R.K and H.F.), GM068680 and GM092035
(to X.C.), AI030162 (to J.T.B.), Georgia Cancer Coalition (to F.R.K. and H.F.),
Georgia Research Alliance (to X.C. and H.F.), and Chinese Academy of Sciences
K. C. Wong Awards (to L.Z. and H.F.).
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