Article

Expression of cytokeratin 10 protein in the human testis showing normal and abnormal spermatogenesis.

Department of Zoology, Faculty of Science, Sohag University, Sohag, Egypt.
Ultrastructural Pathology (impact factor: 0.76). 10/2011; 35(5):209-13. DOI:10.3109/01913123.2011.598255 pp.209-13
Source: PubMed

ABSTRACT CK10 is a heterotetramer of type I and two type II keratins. Aim: This study examines the expression pattern of cytokeratin 10 (CK10) in human testis.
CK10 protein expression was examined using immunofluorescense staining methods in 30 human testicular biopsy specimens (normal spermatogenesis, maturation arrest and Sertoli cell only syndrome, 10 cases each) obtained from patients undergoing investigations for infertility.
In the testis showing normal spermatogenesis, CK10 was expressed in the interstitium and in the seminiferous tubules. A strong cytoplasmic expression was seen in the Leydig cells, Sertoli cells, and spermatocytes. In testes showing spermatogenic arrest, weak CK10 protein expression was observed both in the interstitium and seminiferous tubules (some primary spermatocytes). In the testes showing Sertoli cell only syndrome, negligible CK10 staining was seen both in the seminiferous tubules and in the interstitial cells of Leydig.
To the authors' knowledge, this is the first study indicating CK10 expression in the human testis during normal and abnormal spermatogenesis. The varied expression of CK10 in testes showing abnormal spermatogenesis suggests its possible involvement in this process.

0 0
 · 
0 Bookmarks
 · 
60 Views
  • Source
    Article: A comprehensive review of genetics and genetic testing in azoospermia
    Alaa Hamada, Sandro C. Esteves, Ashok Agarwal
    [show abstract] [hide abstract]
    ABSTRACT: Azoospermia due to obstructive and non-obstructive mechanisms is a common manifestation of male infertility accounting for 10-15% of such cases. Known genetic factors are responsible for approximately 1/3 of cases of azoospermia. Nonetheless, at least 40% of cases are currently categorized as idiopathic and may be linked to unknown genetic abnormalities. It is recommended that various genetic screening tests are performed in azoospermic men, given that their results may play vital role in not only identifying the etiology but also in preventing the iatrogenic transmission of genetic defects to offspring via advanced assisted conception techniques. In the present review, we examine the current genetic information associated with azoospermia based on results from search engines, such as PUBMED, OVID, SCIENCE DIRECT and SCOPUS. We also present a critical appraisal of use of genetic testing in this subset of infertile patients.
    Clinics 02/2013; 68(Supplement 1):39-60. · 2.06 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

abnormal spermatogenesis
 
CK10 expression
 
CK10 protein expression
 
cytokeratin 10
 
expression pattern
 
immunofluorescense staining methods
 
Leydig cells
 
negligible CK10 staining
 
normal spermatogenesis
 
patients undergoing investigations
 
possible involvement
 
primary spermatocytes
 
seminiferous tubules
 
Sertoli cell
 
Sertoli cells
 
spermatogenic arrest
 
strong cytoplasmic expression
 
type II keratins
 
varied expression
 
weak CK10 protein expression