Article

Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.

CHRU de Tours, Service de Bactériologie-Virologie, Hôpital Bretonneau, Tours, France.
Journal of clinical microbiology (Impact Factor: 4.16). 09/2011; 49(11):3943-6. DOI: 10.1128/JCM.00335-11
Source: PubMed

ABSTRACT We evaluated the performances of 4 commercial real-time PCR kits for Bordetella pertussis IS481 sequence detection in nasopharyngeal aspirates by comparison with an in-house real-time PCR assay. Among them, the Simplexa Bordetella pertussis/parapertussis assay (Focus Diagnostics), the SmartCycler Bordetella pertussis/parapertussis assay (Cepheid), and Bordetella R-gene (Argene) present sensitivities over 90%. One kit proved unsuitable for routine clinical use.

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