Autophagy and Aging

Department of Medical Genetics, University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0XY, UK.
Cell (Impact Factor: 32.24). 09/2011; 146(5):682-95. DOI: 10.1016/j.cell.2011.07.030
Source: PubMed


Genetic inhibition of autophagy induces degenerative changes in mammalian tissues that resemble those associated with aging, and normal and pathological aging are often associated with a reduced autophagic potential. Pharmacological or genetic manipulations that increase life span in model organisms often stimulate autophagy, and its inhibition compromises the longevity-promoting effects of caloric restriction, Sirtuin 1 activation, inhibition of insulin/insulin growth factor signaling, or the administration of rapamycin, resveratrol, or spermidine. Here, we discuss the probable cause and effect relationship between perturbed autophagy and aging, as well as possible molecular mechanisms that may mediate the anti-aging effects of autophagy.

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Available from: Guillermo Mariño, Jan 08, 2014
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    • "PK signaling as well as mTOR ( mammalian Target Of Rapamycin ) inhibition ( Araki et al . , 2009 ; Kim et al . , 2012 ; Rolf et al . , 2013 ) , all of which also control autophagy ( Jung et al . , 2010 ) . Indeed , T cell Evidence for age - related declining levels of autophagy stems largely from lower organisms such as yeast , flies , and worms ( Rubinsztein et al . , 2011 ) . A recent study showed that the polyamine spermi - dine induces autophagy and thereby prolongs life span in model organisms ( Eisenberg et al . , 2009 ) ."
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    ABSTRACT: During infection, CD8+ T cells initially expand then contract, leaving a small memory pool providing long lasting immunity. While it has been described that CD8+ T cell memory formation becomes defective in old age, the cellular mechanism is largely unknown. Autophagy is a major cellular lysosomal degradation pathway of bulk material, and levels are known to fall with age. In this study, we describe a novel role for autophagy in CD8+ T cell memory formation. Mice lacking the autophagy gene Atg7 in T cells failed to establish CD8+ T cell memory to influenza and MCMV infection. Interestingly, autophagy levels were diminished in CD8+ T cells from aged mice. We could rejuvenate CD8+ T cell responses in elderly mice in an autophagy dependent manner using the compound spermidine. This study reveals a cell intrinsic explanation for poor CD8+ T cell memory in the elderly and potentially offers novel immune modulators to improve aged immunity. DOI:
    eLife Sciences 11/2014; 3. DOI:10.7554/eLife.03706 · 9.32 Impact Factor
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    • "The process of autophagy is complex and it has at least three inter-related mechanisms: proteasome mediated (PMA), chaperone mediated (CMA) and macro-autophagy. Macro-autophagy is short lasting and takes place in the first hours, while CMA starts later and is more prolonged [57], [58], and we found markers of these two types in our results increased by trehalose treatment. Nevertheless the prolonged up-regulation of autophagy pathway has to be treated with caution because enhanced mitochondrial turnover could be harmful [59]. "
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    ABSTRACT: In this work we investigate the role of CHIP in a new CHIP-mutation related ataxia and the therapeutic potential of trehalose. The patient's fibroblasts with a new form of hereditary ataxia, related to STUB1 gene (CHIP) mutations, and three age and sex-matched controls were treated with epoxomicin and trehalose. The effects on cell death, protein misfolding and proteostasis were evaluated. Recent studies have revealed that mutations in STUB-1 gene lead to a growing list of molecular defects as deregulation of protein quality, inhibition of proteasome, cell death, decreased autophagy and alteration in CHIP and HSP70 levels. In this CHIP-mutant patient fibroblasts the inhibition of proteasome with epoxomicin induced severe pathophysiological age-associated changes, cell death and protein ubiquitination. Additionally, treatment with epoxomicin produced a dose-dependent increase in the number of cleaved caspase-3 positive cells. However, co-treatment with trehalose, a disaccharide of glucose present in a wide variety of organisms and known as a autophagy enhancer, reduced these pathological events. Trehalose application also increased CHIP and HSP70 expression and GSH free radical levels. Furthermore, trehalose augmented macro and chaperone mediated autophagy (CMA), rising the levels of LC3, LAMP2, CD63 and increasing the expression of Beclin-1 and Atg5-Atg12. Trehalose treatment in addition increased the percentage of immunoreactive cells to HSC70 and LAMP2 and reduced the autophagic substrate, p62. Although this is an individual case based on only one patient and the statistical comparisons are not valid between controls and patient, the low variability among controls and the obvious differences with this patient allow us to conclude that trehalose, through its autophagy activation capacity, anti-aggregation properties, anti-oxidative effects and lack of toxicity, could be very promising for the treatment of CHIP-mutation related ataxia, and possibly a wide spectrum of neurodegenerative disorders related to protein disconformation.
    PLoS ONE 09/2014; 9(9):e106931. DOI:10.1371/journal.pone.0106931 · 3.23 Impact Factor
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    • "Resveratrol also shows antioxidant properties as it directly scavenges free radicals and promotes the function of enzymatic antioxidants in cells. Resveratrol has also been shown to improve mitochondrial activity and stimulate autophagy through the activation of the AMPK pathways involving PGC-1 [23]–[25]. "
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    ABSTRACT: Mitochondria form a reticulum network dynamically fuse and divide in the cell. The balance between mitochondria fusion and fission is correlated to the shape, activity and integrity of these pivotal organelles. Resveratrol is a polyphenol antioxidant that can extend life span in yeast and worm. This study examined mitochondria dynamics in replicative senescent yeast cells as well as the effects of resveratrol on mitochondria fusion and fission. Collecting cells by biotin-streptavidin sorting method revealed that majority of the replicative senescent cells bear fragmented mitochondrial network, indicating mitochondria dynamics favors fission. Resveratrol treatment resulted in a reduction in the ratio of senescent yeast cells with fragmented mitochondria. The readjustment of mitochondria dynamics induced by resveratrol likely derives from altered expression profiles of fusion and fission genes. Our results demonstrate that resveratrol serves not only as an antioxidant, but also a compound that can mitigate mitochondria fragmentation in replicative senescent yeast cells.
    PLoS ONE 08/2014; 9(8):e104345. DOI:10.1371/journal.pone.0104345 · 3.23 Impact Factor
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