Article

Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles.

Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Biomaterials (impact factor: 7.4). 08/2011; 32(34):9089-99. DOI:10.1016/j.biomaterials.2011.08.011
Source: PubMed

ABSTRACT Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles' hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles' surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.

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    Hélder A Santos, Luis M Bimbo, Barbara Herranz, Mohammad-Ali Shahbazi, Jouni Hirvonen, Jarno Salonen
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Keywords

advantageous drug
 
Caco-2 cell monolayers
 
coat thermally hydrocarbonized-PSi microparticles
 
coating stability assessment
 
dissolution rate
 
Drug dissolution
 
drug loading degree
 
enhanced drug permeation
 
HFBII content adsorbed
 
HFBII-coating desorbs
 
HT-29 cells
 
hydrophobic materials
 
hydrophobicity
 
hydrophobin II
 
particles' hydrophobicity
 
Porous silicon
 
protein assay
 
release rate
 
self-assembling proteins
 
viability studies