Studies on pH and thermal stability of novel purified L-asparaginase from Pectobacterium carotovorum MTCC 1428
Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.Mikrobiologiia 06/2011; 80(3):349-55. DOI: 10.1134/S0026261711030143
Glutaminase free L-asparaginase is known to be an excellent anticancer agent. In the present study, the combined effect of pH and temperature on the performance of purified novel L-asparaginase from Pectobacterium carotovorum MTCC 1428 was studied under assay conditions using response surface methodology (RSM). Deactivation studies and thermodynamic parameters of this therapeutically important enzyme were also investigated. The optimum pH and temperature of the purified L-asparaginase were found to be 8.49 and 39.3 degrees C, respectively. The minimum deactivation rate constant (k(d)) and maximum half life (t1/2) were found to be 0.041 min(-1) and 16.9 h, respectively at pH of 8.6 and 40 degreesC. Thermodynamic parameters (deltaG, deltaH, deltaS, and activation energies) were also evaluated for purified L-asparaginase. The probable mechanism of deactivation of purified L-asparaginase was explained to an extent on the basis of deactivation studies and thermodynamic parameters.
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ABSTRACT: The word cancer is a silent killer that creeps up on us. Cancer causes a total of 8.2 million deaths in worldwide. Secondary metabolites mainly enzymes are upgraded molecules for cancer therapy. A family of enzymes using for the treatment of human cancers is referred to as L-asparaginase. It hydrolyses the free aspergine to aspartic acid and ammonia. It is well accepted enzyme as a chemotherapeutic agent and antitumor activity against acute lymphoblastic leukemia and lymphosarcoma. There are several therapeutic asparaginase present in the market, but recent discoveries have indicated that the L-asparaginase from marine microbes might be more efficient and also to exhibit lesser side effects. So, the need for new therapeutic enzymes is of great interest in both biotechnology and medicine. This paper comprises a brief introduction about the enzyme, its sources, mechanism of action and as well as a detailed information about its applications.International Journal of Pharmaceutical Sciences Review and Research 07/2015; 33(2):228-234.
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