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Comparison of multilocus variable-number tandem-repeat analysis and multilocus sequence typing for differentiation of hemolytic-uremic syndrome-associated Escherichia coli (HUSEC) collection strains.

Christian Jenke, Björn Arne Lindstedt, Dag Harmsen, Helge Karch, Lin Thorstensen Brandal, Alexander Mellmann

Institute of Hygiene and National Consulting Laboratory for Hemolytic Uremic Syndrome, University Hospital Münster, Robert-Koch-Strasse 41, 48149 Münster, Germany.

Journal Article: Journal of clinical microbiology (impact factor: 4.16). 08/2011; 49(10):3644-6. DOI: 10.1128/JCM.05035-11

Abstract

Multilocus variable-number tandem-repeat analysis (MLVA) was compared to multilocus sequence typing (MLST) to differentiate hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli strains. Although MLVA--like MLST--was highly discriminatory (index of diversity, 0.988 versus 0.984), a low level of concordance demonstrated the limited ability of MLVA to reflect long-term evolutionary events.

Source: PubMed

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Keywords

differentiate hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli strains
 
limited ability
 
MLST
 
Multilocus variable-number tandem-repeat analysis