Article

Sequencing circulating miRNA in maternal plasma with modified library preparation.

Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China.
Clinica chimica acta; international journal of clinical chemistry (impact factor: 2.54). 10/2011; 412(21-22):1989-94. DOI:10.1016/j.cca.2011.07.010 pp.1989-94
Source: PubMed

ABSTRACT Circulating microRNAs in maternal plasma as one type of the cell free nucleotide acid revealed its potential for non-invasive prenatal diagnosis. The next generation sequencing technology provides promising approach detecting miRNA for such purpose.
In this present study, a modified library preparation method for SOLiD sequencing technology was developed and maternal plasma miRNA from single and twin pregnancies was analyzed. Quantitative PCR was carried out for comparison.
Results showed that the sequenced data was improved remarkably with this modified library preparation method; different types and levels of miRNA expression were found in twin pregnancy compared with control. Several miRNAs were validated that remarkably changed in twin pregnancy.
It is indicated that miRNAs might involve the process of pregnancy such as the generation of twin pregnancy, and it also suggested that the specific miRNAs could act as potential biomarkers for clinical diagnosis and therapy.

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Keywords

cell free nucleotide acid
 
Circulating microRNAs
 
different types
 
maternal plasma
 
maternal plasma miRNA
 
miRNAs
 
modified library preparation method
 
next generation sequencing technology
 
non-invasive prenatal diagnosis
 
potential biomarkers
 
promising approach detecting miRNA
 
sequenced data
 
specific miRNAs
 
twin pregnancies
 
twin pregnancy
 

Qinyu Ge