He Z, Piceno Y, Deng Y, Xu M, Lu Z, DeSantis T et al. The phylogenetic composition and structure of soil microbial communities shifts in response to elevated carbon dioxide. ISME J 6: 259-272

Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
The ISME Journal (Impact Factor: 9.3). 07/2011; 6(2):259-72. DOI: 10.1038/ismej.2011.99
Source: PubMed


One of the major factors associated with global change is the ever-increasing concentration of atmospheric CO(2). Although the stimulating effects of elevated CO(2) (eCO(2)) on plant growth and primary productivity have been established, its impacts on the diversity and function of soil microbial communities are poorly understood. In this study, phylogenetic microarrays (PhyloChip) were used to comprehensively survey the richness, composition and structure of soil microbial communities in a grassland experiment subjected to two CO(2) conditions (ambient, 368 p.p.m., versus elevated, 560 p.p.m.) for 10 years. The richness based on the detected number of operational taxonomic units (OTUs) significantly decreased under eCO(2). PhyloChip detected 2269 OTUs derived from 45 phyla (including two from Archaea), 55 classes, 99 orders, 164 families and 190 subfamilies. Also, the signal intensity of five phyla (Crenarchaeota, Chloroflexi, OP10, OP9/JS1, Verrucomicrobia) significantly decreased at eCO(2), and such significant effects of eCO(2) on microbial composition were also observed at the class or lower taxonomic levels for most abundant phyla, such as Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Acidobacteria, suggesting a shift in microbial community composition at eCO(2). Additionally, statistical analyses showed that the overall taxonomic structure of soil microbial communities was altered at eCO(2). Mantel tests indicated that such changes in species richness, composition and structure of soil microbial communities were closely correlated with soil and plant properties. This study provides insights into our understanding of shifts in the richness, composition and structure of soil microbial communities under eCO(2) and environmental factors shaping the microbial community structure.

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    • "It is therefore important to understand the effects of land degradation on soil microbial community composition. Molecular profiling approaches have been widely used to describe microbial diversity in different habitat conditions (Mishra et al. 2013), such as in different soils (Zhang and Xu 2008; He et al. 2012) and extreme environments (Bahl et al. 2011; Chan et al. 2013). Terminal restriction fragment length polymorphism (T-RFLP) has been reported as a rapid, reproducible, and robust molecular technique than most other PCRbased methods for the study of microbial community structure and dynamics (Thies 2007). "
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