Article

Validation of a diagnostic microarray for human papillomavirus: coverage of 102 genotypes.

Department of Dermatology, University of California San Francisco, San Francisco, CA 94143, USA.
Journal of nucleic acids 01/2011; 2011:756905. DOI: 10.4061/2011/756905
Source: PubMed

ABSTRACT Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.

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