Rapid mRNA-Display Selection of an IL-6 Inhibitor Using Continuous-Flow Magnetic Separation

Department of Molecular and Medical Pharmacology, University of California, Los Angeles (USA).
Angewandte Chemie International Edition (Impact Factor: 11.26). 08/2011; 50(36):8295-8. DOI: 10.1002/anie.201101149
Source: PubMed


Even flow: Microfluidics technology can rapidly generate affinity reagents that bind to protein targets using mRNA display. The continuous-flow magnetic separation (CFMS) method (see scheme; steps a-h) offers reproducible means to impose stringent selection conditions without the need for a specialized device. The method yields approximately a 30-fold greater partition efficiency over conventional methods when using interleukin-6 as a model target.

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