Article

Exploiting the interactions of PNA-DNA films with Ni2+ ions: detection of nucleobase mismatches and electrochemical genotyping of the single-nucleotide mismatch in apoE 4 related to Alzheimer's disease.

Department of Chemistry, Beijing Normal University, Beijing, China.
Biosensors & bioelectronics (impact factor: 5.43). 09/2011; 27(1):187-91. DOI:10.1016/j.bios.2011.06.013 pp.187-91
Source: PubMed

ABSTRACT The presence of Ni(2+) enables us to distinguish the presence of single-nucleotide mismatches in PNA (peptide nucleic acids)-DNA films on gold electrodes by electrochemical impedance spectroscopy (EIS). With the help of a modified Randles' equivalent circuit, differences in the charge transfer resistance (ΔR(CT)) before and after the addition of Ni(2+) are a diagnostic measure for the presence of single-nucleotide mismatch. The approach works under real-life conditions with concentrations of the DNA target strand down to 10 fM, and a PNA capture probe is used to genotype the single-nucleotide mismatch in apoE 4 related to Alzheimer's disease (AD).

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Keywords

apoE 4
 
approach works
 
charge transfer resistance
 
diagnostic measure
 
distinguish
 
genotype
 
gold electrodes
 
modified Randles' equivalent circuit
 
peptide nucleic acids)-DNA films
 
PNA
 
PNA capture probe
 
real-life conditions