Worldwide Distribution of Major Clones of Listeria monocytogenes

Institut Pasteur, Paris, France.
Emerging Infectious Diseases (Impact Factor: 7.33). 06/2011; 17(6):1110-2. DOI: 10.3201/eid/1706.101778
Source: PubMed

ABSTRACT Listeria monocytogenes is worldwide a pathogen, but the geographic distribution of clones remains largely unknown. Genotyping of 300 isolates from the 5 continents and diverse sources showed the existence of few prevalent and globally distributed clones, some of which include previously described epidemic clones. Cosmopolitan distribution indicates the need for genotyping standardization.

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    Preventive Veterinary Medicine 11/2014; 118(2-3). DOI:10.1016/j.prevetmed.2014.11.004 · 2.51 Impact Factor
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    ABSTRACT: AimsListeriosis is a frequent silage-associated disease in ruminants. The slugs Arion vulgaris are invaders in gardens, vegetable crops and meadows for silage production. Field and laboratory studies were conducted to clarify whether slugs could host Listeria monocytogenes and thereby constitute a threat to animal feed safety.Methods and ResultsSelective culture of L. monocytogenes from 79 pooled slug samples (710 slugs) resulted in 43% positive, 16% with mean L. monocytogenes values of 405 CFU g−1 slug tissues. Of 62 individual slugs cultured, 11% also tested positive from surface/mucus. Multilocus sequence typing analysis of 36 isolates from different slug pools identified 20 sequence types belonging to L. monocytogenes lineages I and II. Slugs fed ≅4·0 × 105 CFUL. monocytogenes, excreted viable L. monocytogenes in faeces for up to 22 days. Excretion of L. monocytogenes decreased with time, although there were indications of a short enrichment period during the first 24 h.Conclusions Arion vulgaris may act as a vector for L. monocytogenes.Significance and Impact of the StudyHighly slug-contaminated grass silage may pose a potential threat to animal feed safety.
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