Antibacterial Polyketides from the Jellyfish-Derived Fungus Paecilomyces variotii

College of Pharmacy, Pusan National University, Busan 609-735, Korea.
Journal of Natural Products (Impact Factor: 3.8). 07/2011; 74(8):1826-9. DOI: 10.1021/np200350b
Source: PubMed

ABSTRACT Four new polyketides (1-4) were isolated from the fungus Paecilomyces variotii, which was derived from the jellyfish Nemopilema nomurai. The planar structures and relative configurations of these polyketides were elucidated on the basis of spectroscopic analyses, including 2D NMR experiments. The compounds showed inhibitory activity against pathogenic bacteria including methicillin-resistant Staphylococcus aureus 3089 and multi-drug-resistant Vibrio parahemolyticus 7001 with MIC values in the range 5-40 μg/mL.

Download full-text


Available from: Eun La Kim, Sep 14, 2014
59 Reads
  • Source
    • "is fungus has been reported to possess antimicrobial activity against Enterococcus faecalis ( Olivera et al . 2009 ) . A phyto - toxin , 14 - hydroxycornexistin was isolated from this fungus and was found to be active against weeds ( Fields et al . 1996 ) . Further , polyketides isolated from Paecilomyces variotii showed antibacterial activity . ( Juan et al . 2011 ) . This is the first report on free scavenging and its effect as liver protective in CCl 4 induced hepatotoxicity of Paecilomyces variotii . The activity of TRF - 1 may be attributed to the secondary metabolites of fungus Paecilomyces variotti Bain which are yet to be explored ."
    [Show abstract] [Hide abstract]
    ABSTRACT: Endophytic fungi are a rich source of novel organic compounds with interesting biological activities and a high level of biodiversity. In the present investigation, an attempt has been made to isolate, characterize and screen the Tulsi (Ocimum sanctum Linn.) root fungal endophytic fractions (TRF) for hepatoprotective and antioxidant activities. Endophytic fungi from Ocimum sanctum Linn. roots were isolated and cultured aseptically in PDA media. The full fledged grown fungus cultures were fermented in potato dextrose broth and fractionated using ethyl acetate. TRF-1 and TRF-2 were subjected free radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl, hydroxyl free radicals and reducing power assay. TRF-1 was found to be potential free radical scavenger with IC50 values of 71.83 μg/ml for DPPH and 110.85 μg/ml for hydroxyl radical respectively. TRF-1 and TRF-2 were further assessed for hepatoprotective activity in CCl4 induced hepatotoxicity in-vivo. Results showed that TRF-1 (200 mg/kg p.o.) and TRF-2 (200 mg/kg p.o.) significantly reversed the actions of carbon tetrachloride induced hepatotoxicity, as evident by restoration of serum AST, ALT, ALP, bilirubin, triglycerides and protein level to normal values as compared to CCl4 treated group. Restoration of altered LPO (p < 0.001), GSH (p < 0.01) and catalase (p < 0.01) by TRF-1 suggests that the hepatoprotective activity may be attributed to its antioxidant action. This was further supported by histopathological studies. TRF-1 was subjected to rDNA sequencing studies by PCR technique. Based on the morphological and molecular characterization, the fungus was identified as Paecilomyces variotti Bain. Therefore, endophytes from medicinal plants represent great potential for the discovery of new pharmacologically active metabolites.
    Oriental Pharmacy and Experimental Medicine 06/2012; 12(2). DOI:10.1007/s13596-012-0061-7
  • [Show abstract] [Hide abstract]
    ABSTRACT: A highly efficient and accessible synthesis of chiral 3-substituted isoindolinone frameworks is described. The synthesis involved the Rh(I)-catalyzed asymmetric arylation of boronic acids to 2-halobenzaldimines and the subsequent Rh(I)-catalyzed intramolecular aminocarbonylation of the resulting 2-halobenzylamines using an aldehyde as the carbonyl source. The method tolerates a variety of functional groups, yielding isoindolinone derivatives in moderate to high yields with high ee-values. In addition, two Rh(I)-catalyzed transformations could be efficiently accomplished in a one-pot sequence to give chiral isoindolinones by the simple addition of a ligand and an aldehyde after the Rh(I)-catalyzed asymmetric arylation.
    The Journal of Organic Chemistry 03/2012; 77(6):2911-23. DOI:10.1021/jo300201g · 4.72 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The asymmetric dihydroxylation (AD) of o-cyano cinnamates and styrene derivatives leads to efficient construction of chiral phthalide frameworks in high optical purities. This unique reaction is characterized by unusual synergism between CN and osmate groups resulting in rate enhancement of the AD process. The method is amply demonstrated by the synthesis and the structural/stereochemical assignment of the natural products.
    Organic & Biomolecular Chemistry 05/2012; 10(18):3655-61. DOI:10.1039/c2ob25409c · 3.56 Impact Factor
Show more