Enhanced production of bioethanol and ultrastructural characteristics of reused Saccharomyces cerevisiae immobilized calcium alginate beads

Department of Wood Science and Landscape Architecture (BK21 Program), Chonnam National University, Gwangju 500-757, Republic of Korea.
Bioresource Technology (Impact Factor: 4.49). 09/2011; 102(17):8191-8. DOI: 10.1016/j.biortech.2011.06.063
Source: PubMed

ABSTRACT Yeast immobilized on alginate beads produced a higher ethanol yield more rapidly than did free yeast cells under the same batch-fermentation conditions. The optimal fermentation conditions were 30°C, pH 5.0, and 10% initial glucose concentration with 2% sodium alginate beads. The fermentation time using reused alginate beads was 10-14 h, whereas fresh beads took 24h, and free cells took 36 h. All bead samples resulted in nearly a 100% ethanol yield, whereas the free cells resulted in an 88% yield. Transmission electron microscopy (TEM) showed that the shortened time and higher yield with the reused beads was due to a higher yeast population per bead as well as a higher porosity. The ultrastructure of calcium alginate beads and the alginate matrix structure known as the "egg-box" model were observed using TEM.

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    • "Different microbial species have been encapsulated in various matrices for different applications. On many, one might mention the encapsulation of the fungal strains for biocontrol and other applications (Lee et al. 2011; Cruz et al. 1998); the thermophilic and methanogenic bacteria encapsulation (Kanasawud et al. 1989) has also been investigated for potential commercial uses. "
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    ABSTRACT: A bacterial strain E21 was isolated from a sample of water collected in the salt lake located close to Ain Salah, Algeria. The analysis of 16S rRNA gene sequence had indicated that the strain had 93 % sequence similarity with the genus Natrialba sp. strain E21 (GenBank, FR750525.1) and was considered extremely halophilic. Production of biosurfactant by the strain E21 with free and entrapped cells was investigated using soluble starch in the saline conditions. Biosurfactant synthesis was followed by measuring the surface tension and emulsifying index 9 days under optimal conditions (40 °C, pH 7). Some diffusional limitations in alginate and agar beads affected the kinetics of biosurfactant production when compared to that obtained with free cells culture. The minimum values of surface tension were 27 and 30 mN m(-1) achieved after 9 days with free and immobilized cells, respectively, while the corresponding maximum E24 values were 65.3 and 62.3 %, respectively. The re-use of bacterial cells along with the limited cell losses provided by the immobilized system might lead to significant reduction of the biosurfactant production cost.
    Extremophiles 09/2013; 17(6). DOI:10.1007/s00792-013-0580-2 · 2.31 Impact Factor
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    • "After the formation of beads, 0.1 M sterile CaCl 2 solution was replaced with 0.05 M sterile CaCl 2 solution and the cells were allowed to harden for 12 h. Beads were then washed with sterile 0.85% NaCl solution to remove nonadherent cells and CaCl 2 ions (Lee et al., 2011; Razmovski and Vučurovic´, 2011). "
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    ABSTRACT: In this study, optimization of ethanol production from carob pod extract was carried out by immobilized Saccharomyces cerevisiae. Results showed that Ca-alginate concentration and the amount of immobilized cells had significant effects on yield. Optimum conditions for ethanol fermentation were determined to be 2% Ca-alginate concentration, 150rpm agitation rate, 5% yeast cells entrapped in beads and pH 5.5. After validation experiments; ethanol concentration, yield, production rate and sugar utilization rate were respectively 40.10g/L, 46.32%, 3.19g/L/h and 90.66%; and the fermentation time was decreased to 24h. In addition, the immobilized cells were shown to be reusable for five cycles, though a decrease in yield was observed. Finally, carob pod extract was used for ethanol fermentation by controlled and uncontrolled pH without any enrichment, and the results suggest that carob extract can be utilized effectively by immobilized-cell fermentation without the use of enrichments to facilitate yeast growth.
    Bioresource Technology 09/2012; 135. DOI:10.1016/j.biortech.2012.09.006 · 4.49 Impact Factor
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    • "However, in SSF processes using lignocellulosic materials such as rice straw, it is extremely difficult to separate the yeast cells from solid residue unless immobilized cells are used. Ethanol production systems using immobilized yeast cells also have an advantage over free-cell systems in that the highly concentrated immobilized cells are entrapped in the carrier material, resulting in higher ethanol productivity from the beginning of the fermentation process (Lee et al., 2011). "
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    ABSTRACT: Repeated-batch simultaneous saccharification and fermentation (SSF) of alkali-treated rice straw using immobilized yeast was developed to produce ethanol. Saccharomyces cerevisiae cells were immobilized by entrapping in photocrosslinkable resin beads, and we evaluated the possibility of its reuse and ethanol production ability. In batch SSF of 20% (w/w) rice straw, the ethanol yields based on the glucan content of the immobilized cells were slightly low (76.9% of the theoretical yield) compared to free cells (85.2% of the theoretical yield). In repeated-batch SSF of 20% (w/w) rice straw, stable ethanol production of approx. 38gL(-1) and an ethanol yield of 84.7% were obtained. The immobilizing carrier could be reused without disintegration or any negative effect on ethanol production ability.
    Bioresource Technology 07/2012; 123:695-8. DOI:10.1016/j.biortech.2012.07.052 · 4.49 Impact Factor
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