Article

Secretion of N-terminal domain of α-dystroglycan in cerebrospinal fluid.

Department of Neurology, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
Biochemical and Biophysical Research Communications (impact factor: 2.48). 07/2011; 411(2):365-9. DOI:10.1016/j.bbrc.2011.06.150 pp.365-9
Source: PubMed

ABSTRACT α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and investigated the secretion of α-DG-N in human cerebrospinal fluid (CSF). Interestingly, we found that a considerable amount of α-DG-N was present in CSF. α-DG-N in CSF was a sialylated glycoprotein with both N- and O-linked glycan. These observations suggest that secreted α-DG-N may be transported via CSF and have yet unidentified effects on the nervous system.

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Keywords

clarify
 
considerable amount
 
CSF
 
cultured cells
 
human cerebrospinal fluid
 
N-
 
N-terminal domain
 
nervous system
 
O-linked glycan
 
secreted α-DG-N
 
sialylated glycoprotein
 
unidentified effects
 
vivo
 
α-DG-N
 
α-Dystroglycan