Serum concentrations in three children with unintentional tetrahydrozoline overdose
Department of Pediatrics, Children's Mercy Hospitals and Clinics, University of Missouri School of Medicine, Kansas City, MO 64108, USA. Clinical Toxicology
(Impact Factor: 3.67).
06/2011; 49(5):434-5. DOI: 10.3109/15563650.2011.586639
Major symptoms can occur from tetrahydrozoline (THZ) overdoses in young children, requiring intensive care management. We report three cases that presented with CNS depression and cardiovascular effects where serum concentrations were performed.
Case 1 ingested an unknown amount of eye drops containing THZ, resulting in altered mental status, bradycardia, hypothermia, and hypotension. Cases 2 and 3 ingested 7.5 mL of eye drops containing THZ. Case 2 presented to the emergency department (ED) without symptoms but became lethargic and bradycardic 90 min after ingestion. By contrast, Case 3 became lethargic 15 min after ingestion and required intubation on arrival to the ED. All children were admitted to ICU for observation and improved within 24 h of ingestion. Urine obtained for drug screening was positive for THZ. Blood was obtained to assess level using gas-chromatography mass-spectrometry (GC-MS). CASE DISCUSSION: Case 1 had plasma levels of 51.4 and 23.6 ng/mL at 7 and 12 h, respectively, after ingestion, revealing a half-life of 4.4 h. Numerous case reports have been published documenting the dangers of ingesting these topical over-the-counter (OTC) products. However, human PK data are not available to help in our understanding of THZ toxicokinetics and disposition in humans after ingestion.
We report three pediatric cases after ingestion of THZ where plasma concentrations were obtained with a calculated half-life of 4.4 h in one case.
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ABSTRACT: Proteomics is a discipline, which is important in today's drug market scenario due to the continuous increase in development and research of biopharmaceuticals for therapeutic and diagnostic purposes. Biopharmaceuticals are protein drugs and need to be analyzed for the protein post-translational modifications, conformational changes and other degradation pathways. There are many techniques developed for analyzing these complex mo-lecules, which also includes many metabolic and chemical isotope-labeling techniques. One such technique is mass spectroscopy (MS) that has served as a major tool for identification and profiling of protein drugs. MS can be used to determine the isotopic composition of an element within a sample. Labeling with stable isotopes can be used for protein quantification also. MS can also work using label-free techniques. MS can operate on a large scale and has a high throughput, and hence it serves as the most powerful tool to address these purposes of ana-lyzing protein drugs. Moreover, MS can be combined with various other techniques like liquid chromatography (LC), gas chromatography (GC) and another MS system to further optimize the results. This review gives the reader an introduction to the biopharmaceuticals, techniques used to assess them, but it mainly focuses on use of mass spectroscopy for assessment of these protein drugs. Here we detail about different mass spectroscopic methods and their applications for protein assessment. It also compares mass spectroscopy to the conventional methods used for these applications.
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ABSTRACT: No information exists on therapeutic or toxic concentrations of tetrahydrozoline, which has been reported to be used in drug facilitated sexual assaults. The primary aim of this investigation was to establish baseline therapeutic serum and urine concentrations in a sample of healthy volunteers.
Ten healthy volunteers consented to have two drops of Original Visine® eye drops (0.05% tetrahydrozoline solution) placed directly into the conjunctival sac of each eye, 30 s apart, at times 0, 4, 8, and 12 h. Blood and urine samples were then collected at 2, 5, 9, 13, and 24 h post-application and analyzed for concentrations. Tetrahydrozoline concentrations are described using measures of central tendency and dispersion at each time point, with predictions of serum and urine concentrations over time calculated using a linear mixed effects regression model.
Tetrahydrazoline concentrations were detectable in both serum and urine after therapeutic ocular administration. The mean serum half-life of tetrahydrozoline was approximately 6 h. Systemic absorption varied among subjects, with the maximum serum concentrations ranging from 0.068 to 0.380 ng/ml. At 24 h, all patients had detectable urine concentrations of tetrahydrozoline (range = 13?210 ng/ml).
When used as directed by the manufacturer for therapeutic ocular administration, tetrahydrozoline concentrations were detectable in both serum and urine up to 12 h after the last administered dose. A concentration greatly exceeding the 95% confidence interval of drug present during therapeutic ocular use may be suggestive of illegal adulterant use or accidental or suicidal overdose.
Clinical Toxicology 11/2011; 49(9):810-4. DOI:10.3109/15563650.2011.615064 · 3.67 Impact Factor
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ABSTRACT: We report two cases of drug-facilitated sexual assault which presented with CNS depression rendering the victims vulnerable to sexual assault and unable to recall the events surrounding the crimes. In each tetrahydrozoline (THZ) was quantified.
Case #1 ingested an unknown amount of eye drops containing THZ mixed with alcoholic beverages. Case #1 presented to the emergency department (ED) approximately 7h after reportedly being sexually assaulted and was without symptoms in the ED. Earlier reports from individuals who saw her indicated the victim to be "heavily intoxicated". Case #2 also ingested an unknown amount of eye drops containing THZ mixed with alcoholic beverages. Case #2 presented to an ED without symptoms approximately 23h after reportedly being sexually assaulted. Urine obtained from both cases was assessed for THZ concentrations using gas-chromatography-mass spectrometry. CASE DISCUSSION: Case #1 had a 0.15g% (by weight by volume) urine ethanol concentration and a urinary THZ concentration of 1.481ng/ml, approximately 7h after ingestion. Case #2 was negative for ethanol but had a urine THZ concentration of 108ng/ml at 23h post-ingestion. Few case reports have been published documenting the use of THZ to facilitate sexual assault.
We report two cases of drug-facilitated sexual assault involving the use of THZ where alcoholic beverages were concomitantly consumed. This is the first paper to our knowledge which discusses pharmacology of THZ ingestion and the significance of alcohol being present.
Forensic science international 05/2012; 221(1-3):e12-6. DOI:10.1016/j.forsciint.2012.04.004 · 2.14 Impact Factor
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