Investigation of Neuropsychopharmacological Effects of a Polyherbal Formulation on the Learning and Memory Process in Rats

Department of Pharmacology, Shri Sarvajanik Pharmacy College, Mehsana, India.
Journal of Young Pharmacists 04/2011; 3(2):119-24. DOI: 10.4103/0975-1483.80296
Source: PubMed


To investigate the neuropsychopharmacological effect of a polyherbal formulation (PHF) on the learning and memory processes in rats.
PHF contains Withania somnifera (Ashwagandha), Nardostachys jatamansi (Jatamansi), Rauwolfia serpentina (Sarpagandha), Evolvulus alsinoides (Shankhpushpi), Asparagus racemosus (Shatavari), Emblica officinalis (Amalki), Mucuna pruriens (Kauch bij extract), Hyoscyamus niger (Khurasani Ajmo), Mineral resin (Shilajit), Pearl (Mukta Shukhti Pishti), and coral calcium (Praval pishti). Its effect (500 mg / kg, p.o.) on the learning and memory processes was tested. The activity of PHF on memory acquisition and retention was studied using passive avoidance learning and elevated plus maze model (EPM) in rats.
The animals treated with PHF showed a significant decrease in transfer latency as compared to the control group in EPM. PHF also produced significant improvement in passive avoidance acquisition and memory retrieval, as compared to the controls and reduced the latency to reach the shock free zone (SFZ) after 24 hours.
The PHF produces significant improvement in passive avoidance acquisition and memory retrieval in rats, which needs further investigation.

12 Reads
  • Source
    • "Among these, the most important plant is Ashwagandha whose extracts make a significant component to the daily supplements for body and brain health. Although a variety of Ashwagandha extracts have displayed neuroprotective, neuroregenerative and anticancer potentials in recent in vitro studies [7], [8], [9], [10], [11] using brain-derived cells, potentials of water extract of leaves of Ashwagandha (ASH-WEX) remain largely unexplored. In the present study, we used glutamate induced excitotoxicity as a model to investigate the neuroprotective potentials of ASH-WEX. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Glutamate neurotoxicity has been implicated in stroke, head trauma, multiple sclerosis and neurodegenerative disorders. Search for herbal remedies that may possibly act as therapeutic agents is an active area of research to combat these diseases. The present study was designed to investigate the neuroprotective role of Withania somnifera (Ashwagandha), also known as Indian ginseng, against glutamate induced toxicity in the retinoic acid differentiated rat glioma (C6) and human neuroblastoma (IMR-32) cells. The neuroprotective activity of the Ashwagandha leaves derived water extract (ASH-WEX) was evaluated. Cell viability and the expression of glial and neuronal cell differentiation markers was examined in glutamate challenged differentiated cells with and without the presence of ASH-WEX. We demonstrate that RA-differentiated C6 and IMR-32 cells, when exposed to glutamate, undergo loss of neural network and cell death that was accompanied by increase in the stress protein HSP70. ASH-WEX pre-treatment inhibited glutamate-induced cell death and was able to revert glutamate-induced changes in HSP70 to a large extent. Furthermore, the analysis on the neuronal plasticity marker NCAM (Neural cell adhesion molecule) and its polysialylated form, PSA-NCAM revealed that ASH-WEX has therapeutic potential for prevention of neurodegeneration associated with glutamate-induced excitotoxicty.
    PLoS ONE 05/2012; 7(5):e37080. DOI:10.1371/journal.pone.0037080 · 3.23 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Passive avoidance conditioning is analyzed in a three compartment apparatus that consists of a light compartment, a dark dangerous compartment in which foot shock was delivered, and a dark safe one where the rats were not shocked. It is concluded that latency increase of passive response is caused not by memory of the shock in a strictly certain location and, accordingly, not by shock avoidance in it, but by non-specific defensive response (freezing) unrelated to the shock location.
    Moscow University Biological Sciences Bulletin 04/2013; 68(2). DOI:10.3103/S009639251302003X
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Passive avoidance conditioning is analyzed in a three-compartment apparatus comprising the central light compartment, the dark dangerous compartment in which the electric foot shock was delivered, and the dark safe compartment where the rats were not punished. The passive avoidance performance was characterized, in addition to the latent period duration, by the number of visits into the safe compartment. The experimental data indicate that the latency of the passive avoidance response and the safe compartment preference obey different laws. The latency depends linearly on the number of shocks, while neither the safe compartment preference nor its relation to the number of shocks was observed. Piracetam had no effect on the latency, but enhanced the number of visits into the safe compartment. It is concluded that this modified model may be useful in an analysis of nootropic effects of neuropsychotropic substances.
    Eksperimental'naia i klinicheskaia farmakologiia 01/2007; 70(2):67-9.
Show more


12 Reads
Available from