Effects of Root and Stem Extracts of Asparagus cochinchinensis on Biochemical Indicators Related to Aging in the Brain and Liver of Mice
ABSTRACT Asparagus cochinchinensis is a traditional Chinese medicine used for treating lung and spleen-related diseases. In this study, we compared the medicinal effects of A. cochinchinensis root and stem extracts on the activity of superoxide dismutase (SOD), the content of malonaldehyde (MDA) and total protein content in the brain, liver and plasma of mice. Polysaccharides and aqueous extracts of the roots significantly increased the spleen index and the SOD activity but reduced the MDA content and slowed down the aging process. In contrast, feeding with the stem extracts significantly reduced the SOD activity and increased the MDA accumulation in the brain and liver of mice, suggesting that the stem extracts may not be appropriate for treating aging-related diseases.
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ABSTRACT: Colla corii asini (E'jiao), donkey-hide gelatin prepared by stewing and concentrating from Equus asinus L. donkey hide, is a traditional Chinese medicine preparation widely used in clinical hematic antanemic therapy in China. The aim of the present study was to investigate potential anti-aging effect of Colla corii asini and explore related mechanisms in D-galactose (gal) induced aging model mice. The mice were artificially induced aging by subcutaneously injection with D-gal at the dose of 100 mg/kg·d for 8 weeks. Colla corii asini was simultaneously treated to them once daily by intragastric gavage. Appetite, mental condition, body weight, and organ index were observed. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), as well as levels of malondialdehyde (MDA) in serum, brain, and liver were determined by according assay kits. Western blotting analysis was used to detect p16 and p21 expression. Results indicated that Colla corii asini could improve appetite, mental condition, body weight, and organ condition of model mice, improve SOD, CAT, and GSH-Px activities, reduce MDA levels, and modulate age-related genes expression in D-gal induced mice. Therefore, Colla corii asini may have effect to suppress the aging process through enhancing antioxidant activity, scavenging free radicals, and modulating aging-related gene expression.Biological & Pharmaceutical Bulletin 01/2012; 35(12):2128-32. DOI:10.1248/bpb.b12-00238 · 1.78 Impact Factor
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ABSTRACT: In this study, we evaluated the anti-inflammatory effect of PM014 on cigarette smoke induced lung disease in the murine animal model of chronic obstructive pulmonary disease (COPD). Mice were exposed to cigarette smoke (CS) for 2 weeks to induce COPD-like lung inflammation. Two hours prior to cigarette smoke exposure, the treatment group was administered PM014 via an oral injection. To investigate the effects of PM014, we assessed PM014 functions in vivo, including immune cell infiltration, cytokine profiles in bronchoalveolar lavage (BAL) fluid and histopathological changes in the lung. The efficacy of PM014 was compared with that of the recently developed anti-COPD drug, roflumilast. PM014 substantially inhibited immune cell infiltration (neutrophils, macrophages, and lymphocytes) into the airway. In addition, IL-6, TNF-alpha and MCP-1 were decreased in the BAL fluid of PM014-treated mice compared to cigarette smoke stimulated mice. These changes were more prominent than roflumilast treated mice. The expression of PAS-positive cells in the bronchial layer was also significantly reduced in both PM014 and roflumilast treated mice. These data suggest that PM014 exerts strong therapeutic effects against CS induced, COPD-like lung inflammation. Therefore, this herbal medicine may represent a novel therapeutic agent for lung inflammation in general, as well as a specific agent for COPD treatment.BMC Complementary and Alternative Medicine 09/2013; 13(1):219. DOI:10.1186/1472-6882-13-219 · 1.88 Impact Factor
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ABSTRACT: Objective Yintian Granule (YTG), as a type of local preparation and applied for Chinese patent, is mainly composed of several traditional Chinese herbs used as both drug and food such as Lonicera macranthoides, Gardenia jasminoides, and Asparagus cochinchinenis, and has been reported to demonstrate the beneficial effects on human health in other researches. In this paper, the protective effects of YTG against experimental acute liver injury of mice were investigated to assess the value of this innovative Chinese herbal compound. Methods Carbon tetrachloride (CCl4) and 50% ethanol were used respectively to induce the acute liver injury model in mice pre-administered with YTG. Lai's method was used to detect the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, Coomassie brilliant blue method was used for the determination of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, and hematoxylin-eosin (HE) staining was used for the observation of liver histomorphometry. Results YTG significantly lowered the elevated ALT and AST levels, increased the SOD activity, decreased the MDA content, and inhibited the deterioration of liver. Conclusion YTG exerts protective effected against hepatocyte damage in mice induced by CCl4 and 50% ethanol, respectively.08/2014; 6(3):247–252. DOI:10.1016/S1674-6384(14)60036-8