In vitro evaluation on the antioxidant capacity of triethylchebulate, an aglycone from Terminalia chebula Retz fruit

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
Indian Journal of Pharmacology (Impact Factor: 0.69). 05/2011; 43(3):320-3. DOI: 10.4103/0253-7613.81508
Source: PubMed


To evaluate the antioxidant and free-radical scavenging activities of triethylchebulate (TCL), an aglycone isolated from the fruit of Terminalia chebula Retz.
Microsomes, mitochondria and red blood cells (RBCs) were isolated from rat liver. The antioxidant capacities were evaluated by determining the inhibitory effects of TCL on lipid peroxidation, hydrogen peroxide (H(2)O(2))-induced RBCs hemolysis and RBCs autoxidative hemolysis. The free-radical scavenging activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and 2´,7´-dichlorodihydrofluorescin diacetate (DCFH(2)-DA) assay.
TCL significantly inhibited FeSO(4)/Cys-induced microsomes lipid peroxidation and protected both H(2)O(2-)-induced RBCs hemolysis and RBCs auto-hemolysis in a dose-dependent manner. Furthermore, TCL demonstrated potent DPPH free-radical scavenging ability with IC(50) at 2.4×10(-5) M. In addition, TCL also moderately suppressed azide-induced mitochondria ROS formation.
These results demonstrated that TCL was a strong antioxidant and free-radical scavenger, which might contribute to the anti-oxidative ability of Terminalia chebula Retz.

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    • "Strong antioxidant activity of aqueous extract of T. chebula was observed by studying the inhibition of radiation induced lipid peroxidation in rat liver microsomes at different doses [28] , and methanolic extract was also found to inhibit lipid peroxide formation and to scavenge hydroxyl and superoxide radicals in vitro [29] . Acetone extract has stronger antioxidant activity than alphatocopherol and HPLC analysis with diode array detection indicated the presence of hydroxybenzoic acid derivatives, hydroxycinnamic acid derivatives, flavonol aglycones and their glycosides, as main phenolic compounds [30] "
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