Platelet-rich plasma, rhOP-1® (rhBMP-7) and frozen rib allograft for the reconstruction of bony mandibular defects in sheep. A pilot experimental study
Research Unit FREMAP Hospital, Ctra Pozuelo 61, 28220 Majadahonda, Madrid, Spain. Injury
(Impact Factor: 2.14).
12/2009; 40 Suppl 3(Suppl 3):S44-9. DOI: 10.1016/S0020-1383(09)70011-7
A 6 cm bony defect in the mandible of 15 sheep, 8 years old, was reconstructed using variously allograft of frozen rib, rhOP-1 (rh BMP-7), platelet-rich plasma (PRP), and a combination of frozen rib allograft and rhOP-1. The histological, histomorphometric, immunohistochemical and radiographic features of reconstruction were analysed. The animals were euthanised at 2 months postoperatively. In the control and PRP groups, no bone formation was detected. The sheep receiving rhOP-1 showed some and those receiving both rhOP-1 and allograft showed most new bone formation; in both groups this was through endochondral and also fibrous ossification. The combination of bone allograft with growth factors demonstrated osteoconductive as well as osteoinductive properties, and is appealing in the management of problem fractures.
Available from: Yoav Leiser
- "The term platelet-rich plasma (PRP) refers to different types of platelet concentrates obtained using different techniques.57 It is believed that PRP contains growth factors and might therefore have biological properties that could enhance the regeneration of certain tissues.58–61 It was also recently implied to have antimicrobial properties which also contribute to tissue repair and regeneration.62 "
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ABSTRACT: Gradual bone lengthening using distraction osteogenesis principles is the gold standard for the treatment of hypoplastic facial bones. However, the long treatment time is a major disadvantage of the lengthening procedures. The aim of this study is to review the current literature and summarize the cellular and molecular events occurring during membranous craniofacial distraction osteogenesis. Mechanical stimulation by distraction induces biological responses of skeletal regeneration that is accomplished by a cascade of biological processes that may include differentiation of pluripotential tissue, angiogenesis, osteogenesis, mineralization, and remodeling. There are complex interactions between bone-forming osteoblasts and other cells present within the bone microenvironment, particularly vascular endothelial cells that may be pivotal members of a complex interactive communication network in bone. Studies have implicated number of cytokines that are intimately involved in the regulation of bone synthesis and turnover. The gene regulation of numerous cytokines (transforming growth factor-β, bone morphogenetic proteins, insulin-like growth factor-1, and fibroblast growth factor-2) and extracellular matrix proteins (osteonectin, osteopontin) during distraction osteogenesis has been best characterized and discussed. Understanding the biomolecular mechanisms that mediate membranous distraction osteogenesis may guide the development of targeted strategies designed to improve distraction osteogenesis and accelerate bone regeneration that may lead to shorten the treatment duration.
01/2014; 2(1):e98. DOI:10.1097/GOX.0000000000000043
Available from: Nicola Maffulli
- "Bioreactors can be chemical, mechanical, electrical, or magnetic, and had demonstrated the ability to promote and accelerate the differentiation process of MSCs into fibroblasts . The chemical stimulation technique consists on the use of growth factors [38-43, 49, 50, 52, 74, 88-90]. The major problem associated with this procedure was the possibility to maintain sufficient quantities of growth factor within the local tissue. "
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ABSTRACT: The anterior cruciate ligament (ACL) is fundamental for the knee joint stability. ACL tears are frequent, especially during sport activities, occurring mainly in young and active patients. Nowadays, the gold standard for the management of ACL tears remains the surgical reconstruction with autografts or allografts. New strategies are being developed to resolve the problems of ligament grafting and promote a physiological healing process of ligamentous tissue without requiring surgical reconstruction. Moreover, these strategies can be applicable in association surgical reconstruction and may be useful to promote and accelerate the healing process. The use of growth factors and stem cells seems to offer a new and fascinating solution for the management of ACL tears. The injection of stem cell and/or growth factors in the site of ligamentous injury can potentially enhance the repair process of the physiological tissue. These procedures are still at their infancy, and more in vivo and in vitro studies are required to clarify the molecular pathways and effectiveness of growth factors and stem cells therapy for the management of ACL tears. This review aims to summarize the current knowledge in the field of growth factors and stem cells for the management of ACL tears.
The Open Orthopaedics Journal 11/2012; 6:525-30. DOI:10.2174/1874325001206010525
Available from: Andreas Schmitt
- "There, great hope was set on regenerative medicine to develop alternative therapies for cartilage damage, arthritis, large bone defects, or atrophic tendon ruptures during the last decade. These are all indications, which are treatable only insufficiently with conventional implants and surgical procedures        . Therefore, they frequently result in decreased function of the musculoskeletal system or even loss of patients' mobility. "
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ABSTRACT: Stem cell research plays an important role in orthopedic regenerative medicine today. Current literature provides us with promising results from animal research in the fields of bone, tendon, and cartilage repair. While early clinical results are already published for bone and cartilage repair, the data about tendon repair is limited to animal studies. The success of these techniques remains inconsistent in all three mentioned areas. This may be due to different application techniques varying from simple mesenchymal stem cell injection up to complex tissue engineering. However, the ideal carrier for the stem cells still remains controversial. This paper aims to provide a better understanding of current basic research and clinical data concerning stem cell research in bone, tendon, and cartilage repair. Furthermore, a focus is set on different stem cell application techniques in tendon reconstruction, cartilage repair, and filling of bone defects.
02/2012; 2012:394962. DOI:10.1155/2012/394962
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