In vitro study of CD133 human stem cells labeled with superparamagnetic iron oxide nanoparticles.
ABSTRACT Superparamagnetic iron oxide nanoparticles (SPIONs) are applied in stem cell labeling because of their high magnetic susceptibility as compared with ordinary paramagnetic species, their low toxicity, and their ease of magnetic manipulation. The present work is the study of CD133+ stem cell labeling by SPIONs coupled to a specific antibody (AC133), resulting in the antigenic labeling of the CD133+ stem cell, and a method was developed for the quantification of the SPION content per cell, necessary for molecular imaging optimization. Flow cytometry analysis established the efficiency of the selection process and helped determine that the CD133 cells selected by chromatographic affinity express the transmembrane glycoprotein CD133. The presence of antibodies coupled to the SPION, expressed in the cell membrane, was observed by transmission electron microscopy. Quantification of the SPION concentration in the marked cells using the ferromagnetic resonance technique resulted in a value of 1.70 x 10(-13) mol iron (9.5 pg) or 7.0 x 10(6) nanoparticles per cell (the measurement was carried out in a volume of 2 muL containing about 6.16 x 10(5) pg iron, equivalent to 4.5 x 10(11) SPIONs).
Chapter: Cancer Stem Cells in Solid Tumors[Show abstract] [Hide abstract]
ABSTRACT: Confirmation and assessment of general applicability of cancer stem cell concept towards solid tumors greatly depends on development of reliable approaches to selectively identify populations of neoplastic cells carrying “stemness” features, such as extensive capacity for self-renewal and ability to undergo a range of differentiation events. This chapter describes such assays as sphere formation, side population isolation and cancer stem cell marker detection and addresses their potential pitfalls. Also discussed are the cell of origin of stem cells and remaining challenges in solid tumor stem cell research. KeywordsBrain–Breast–Cell of origin–Challenges–Colon–Head and neck–Kidney–Liver–Ovary–Pancreas–Prostate–Side population–Skin–Solid tumor–Sphere assay–Stem cell markers12/2009: pages 295-326;
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ABSTRACT: To investigate the kinetics, mechanism and extent of MNP loading into endothelial cells and the effect of this loading on cell function. MNP uptake was examined under field on/off conditions, utilizing varying magnetite concentration MNPs. MNP-loaded cell viability and functional integrity was assessed using metabolic respiration, cell proliferation and migration assays. MNP uptake in endothelial cells significantly increased under the influence of a magnetic field versus non-magnetic conditions. Larger magnetite density of the MNPs led to a higher MNP internalization by cells under application of a magnetic field without compromising cellular respiration activity. Two-dimensional migration assays at no field showed that higher magnetite loading resulted in greater cell migration rates. In a three-dimensional migration assay under magnetic field, the migration rate of MNP-loaded cells was more than twice that of unloaded cells and was comparable to migration stimulated by a serum gradient. Our results suggest that endothelial cell uptake of MNPs is a force dependent process. The in vitro assays determined that cell health is not adversely affected by high MNP loadings, allowing these highly magnetically responsive cells to be potentially beneficial therapy (gene, drug or cell) delivery systems.Pharmaceutical Research 01/2012; 29(5):1270-81. · 4.74 Impact Factor
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ABSTRACT: In the 21st century, nanoscience and nanotechnology obtains the world attention due to this revolutionary theory and technical features. Nanoscience and nanotechnology cover the theory and technology of physics, chemistry, medicine, material science, biomedical engineering and biology, therefore, they have no less contribution to science and technology as biotechnology and information technology. Recent years have witnessed the rapid development of China's nanoscience and nanotechnology with widespread influence. It was attended by scientists of the world. Research, development and application of nanotechnology research in China can be summed up in three characteristics: the first, China government in support of sustainable development; the second, significant academic achievements, and the third, a clear consensus on sustainable development for nanoscience and nanotechnology research and development. In this review paper, we discussed the pharmacology and toxicology of nanomedicines, and presented some issues on research and development and application of nanomedicines in the future.Asian Journal of Pharmacodynamics and Pharmacokinetics. 01/2009; 9:27-49.