Superparamagnetic iron oxide nanoparticles (SPIONs) are applied in stem cell labeling because of their high magnetic susceptibility as compared with ordinary paramagnetic species, their low toxicity, and their ease of magnetic manipulation. The present work is the study of CD133+ stem cell labeling by SPIONs coupled to a specific antibody (AC133), resulting in the antigenic labeling of the CD133+ stem cell, and a method was developed for the quantification of the SPION content per cell, necessary for molecular imaging optimization. Flow cytometry analysis established the efficiency of the selection process and helped determine that the CD133 cells selected by chromatographic affinity express the transmembrane glycoprotein CD133. The presence of antibodies coupled to the SPION, expressed in the cell membrane, was observed by transmission electron microscopy. Quantification of the SPION concentration in the marked cells using the ferromagnetic resonance technique resulted in a value of 1.70 x 10(-13) mol iron (9.5 pg) or 7.0 x 10(6) nanoparticles per cell (the measurement was carried out in a volume of 2 muL containing about 6.16 x 10(5) pg iron, equivalent to 4.5 x 10(11) SPIONs).
[Show abstract][Hide abstract] ABSTRACT: In the 21st century, nanoscience and nanotechnology obtains the world attention due to this revolutionary theory and technical features. Nanoscience and nanotechnology cover the theory and technology of physics, chemistry, medicine, material science, biomedical engineering and biology, therefore, they have no less contribution to science and technology as biotechnology and information technology. Recent years have witnessed the rapid development of China's nanoscience and nanotechnology with widespread influence. It was attended by scientists of the world. Research, development and application of nanotechnology research in China can be summed up in three characteristics: the first, China government in support of sustainable development; the second, significant academic achievements, and the third, a clear consensus on sustainable development for nanoscience and nanotechnology research and development. In this review paper, we discussed the pharmacology and toxicology of nanomedicines, and presented some issues on research and development and application of nanomedicines in the future.
[Show abstract][Hide abstract] ABSTRACT: Confirmation and assessment of general applicability of cancer stem cell concept towards solid tumors greatly depends on development
of reliable approaches to selectively identify populations of neoplastic cells carrying “stemness” features, such as extensive
capacity for self-renewal and ability to undergo a range of differentiation events. This chapter describes such assays as
sphere formation, side population isolation and cancer stem cell marker detection and addresses their potential pitfalls.
Also discussed are the cell of origin of stem cells and remaining challenges in solid tumor stem cell research.
KeywordsBrain–Breast–Cell of origin–Challenges–Colon–Head and neck–Kidney–Liver–Ovary–Pancreas–Prostate–Side population–Skin–Solid tumor–Sphere assay–Stem cell markers
[Show abstract][Hide abstract] ABSTRACT: According to the cancer progression model, several events are required for the progression from normal epithelium to carcinoma. Due to their extended life span, stem cells would represent the most likely target for the accumulation of these genetic events but this has not been formally proven for most of solid cancers. Even more importantly, cancer stem cells seem to harbor mechanisms protecting them from standard cytotoxic therapy. While cancer stem cells have been demonstrated to be responsible for therapy resistance in glioblastoma and pancreatic cancer, further evidence now points to similar mechanisms in colon cancer stem cells. Therefore, it appears reasonable to conclude that there is sufficient evidence now for the existence of cancer stem cells in several epithelial tumors and that these cancer stem cells pose a significant threat via their resistance to standard therapies. Accumulating evidence suggests, however, that novel approaches targeting cancer stem cells are capable of overcoming these resistance mechanisms. To further foster our understanding of in vivo cancer stem cell biology, novel imaging modalities in conjunction with clinically most relevant cancer stem cell models need to be developed and utilized. These studies will then pave the way to better elucidate the underlying regulatory mechanisms of cancer stem cells and develop platforms for targeted theragnostics, which may eventually help improving the prognosis of our patients suffering from these deadly diseases.
Seminars in Cancer Biology 04/2010; 20(2):77-84. DOI:10.1016/j.semcancer.2010.03.004 · 9.33 Impact Factor
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