Article

The TACC proteins: TACC-ling microtubule dynamics and centrosome function

Cell and Developmental Biology Program, Centre for Genomic Regulation (CRG), University Pompeu Fabra (UPF), Dr Aiguader 88, Barcelona 08003, Spain.
Trends in cell biology (Impact Factor: 12.31). 08/2008; 18(8):379-88. DOI: 10.1016/j.tcb.2008.06.005
Source: PubMed

ABSTRACT A major quest in cell biology is to understand the molecular mechanisms underlying the high plasticity of the microtubule network at different stages of the cell cycle, and during and after differentiation. Initial reports described the centrosomal localization of proteins possessing transforming acidic coiled-coil (TACC) domains. This discovery prompted several groups to examine the role of TACC proteins during cell division, leading to indications that they are important players in this complex process in different organisms. Here, we review the current understanding of the role of TACC proteins in the regulation of microtubule dynamics, and we highlight the complexity of centrosome function.

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    • "Prior to this finding , the cytoskeletal functions of TACC3 were well - characterized primarily at the centrosome and mitotic spindle , where TACC3 is necessary for XMAP215 recruitment and astral and spindle MT elongation ( Lee et al . , 2001 ; Peset and Vernos , 2008 ; Lioutas and Vernos , 2013 ; Thakur et al . , 2014 ) . "
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    • "However, despite the well-known localization of XMAP215 at growing MT plus ends (Brouhard et al., 2008), it has been unclear whether vertebrate TACC3 interacts with XMAP215 specifically at plus ends during interphase. There has been some evidence that, in other systems, TACC proteins may bind to and regulate the plus ends of MTs (Peset and Vernos, 2008). Whereas the single TACC orthologue in Drosophila (called D-TACC) is highly concentrated at centrosomes in vivo (Gergely et al., 2000b), green fluorescent protein (GFP)–tagged D- TACC was also observable as small puncta that emanate from the centrosome, likely corresponding to growing MT plus ends (Lee et al., 2001). "
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    • "The peripheries of mitotic cells are marked with dotted outlines; none of the Alp7 mutant proteins localise to SPBs. Sequence comparison of various TACC proteins and substitutions of five amino acids to alanines (boxed) are shown at the bottom (hTACC3, human TACC3; Xl, Xenopus laevis, Xl-TACC3 = Maskin [10]; D, Drosophila melanogaster; "
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