Article

[Interferon-gamma decreases the adhesiveness and invasiveness of prostate cancer cells].

Department of Urology, the Fourth Hospital Affiliated to Harbin Medical University, Harbin, Heilongjiang 150001, China.
Zhonghua nan ke xue = National journal of andrology 06/2008; 14(6):513-6. pp.513-6
Source: PubMed

ABSTRACT To study the effects of interferon-gamma (IFN-gamma) on the adhesiveness and invasiveness of prostate cancer cells.
We determined the effect of IFN-gamma on the adhesiveness of prostate cancer cell lines LNCaP and PC-3 by treating the plate with fibronectin and laminin, on the invasiveness of prostate cancer cells into the artificial basal membrane consisting of Matrigel and fibronectin using the Transwell chamber, and on the expression of annexin-2 in these cells by Western blot.
The adhesion rates of prostate cancer cell lines LNCaP and PC-3 were 21% and 23% in the IFN-gamma treatment group, significantly lower than 46% and 40% in the untreated group (P < 0.05). The invasiveness of the cells was markedly decreased in the former as compared with the latter (P < 0.05). IFN-gamma significantly inhibited the expression of annexin-2 in prostate cancer cell lines LNCaP and PC-3 (P < 0.05).
IFN-gamma can decrease the adhesiveness and invasiveness of the prostate cancer cell line by down-regulating the expression of annexin-2.

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Keywords

adhesion rates
 
adhesiveness
 
artificial basal membrane
 
fibronectin
 
former
 
IFN-gamma
 
IFN-gamma treatment group
 
interferon-gamma
 
lower
 
PC-3
 
prostate cancer cell line
 
prostate cancer cell lines LNCaP
 
prostate cancer cells
 
Transwell chamber
 
Western blot