Article

Establishment of a polymerase chain reaction method for detection of Escherichia coli in amniotic fluid in patients with chorioamnionitis.

Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University Medical School, Nagoya, Japan.
Fetal Diagnosis and Therapy (impact factor: 1.05). 01/2008; 24(2):132-9. DOI:10.1159/000142143 pp.132-9
Source: PubMed

ABSTRACT We investigated whether Escherichia coli could be detected by E. coli of reference (ECOR) grouping and virulence factors (VFs) in amniotic fluid using polymerase chain reaction (PCR).
From 18 patients with clinical symptoms, such as cyclic uterine contraction, genital bleeding and cervical ripening, who subsequently developed abortion or labor before term, and from 40 normal pregnant women undergoing diagnostic amniocentesis, amniotic fluid was obtained. All samples were negative for standard culture. Six patients with symptoms were classified into the ECOR group, and with VFs, E. coli was detected in 6 patients. Thus, 4 patients were positive for both tests.
We could establish a detection method for E. coli in amniotic fluid using both ECOR grouping and VFs with PCR.

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Keywords

18 patients
 
4 patients
 
40 normal pregnant women undergoing diagnostic amniocentesis
 
6 patients
 
amniotic fluid
 
cervical ripening
 
cyclic uterine contraction
 
ECOR
 
ECOR group
 
ECOR grouping
 
Escherichia coli
 
genital
 
patients
 
polymerase chain reaction
 
standard culture
 
VFs
 
virulence factors
 

Ghada A Daoud