Article

Gastrointestinal Pythiosis in 10 Dogs from California

Department of Medicine and Epidemiology, University of California, School of Veterinary Medicine, Davis, CA, USA.
Journal of Veterinary Internal Medicine (Impact Factor: 2.22). 07/2008; 22(4):1065-9. DOI: 10.1111/j.1939-1676.2008.0123.x
Source: PubMed

ABSTRACT Pythium insidiosum is an aquatic oomycete that causes severe segmental thickening of the canine gastrointestinal (GI) tract, resulting in weight loss, vomiting, diarrhea, and death. Infection in dogs previously has been observed primarily in the southeastern United States.
To describe the clinicopathologic and epidemiologic findings associated with GI pythiosis in 10 dogs from California.
Dogs were initially identified on the basis of supportive clinical findings and routine histology. Pythiosis was confirmed in each dog with at least one of the following: immunoblot serology, enzyme-linked immunosorbent assay serology, immunohistochemistry, and culture followed by species-specific polymerase chain reaction, rRNA gene sequencing, or both.
Between September 2003 and December 2006, GI pythiosis was confirmed in 1 dog from central California and 9 dogs that lived within a 30-mile radius of Davis, CA. Seven of 8 dogs for which environmental data were available had frequent access to flooded rice fields or other water sources. Esophageal lesions were present in 2 of 10 dogs. Common laboratory findings included eosinophilia (7/9), hypoalbuminemia (9/9), and hyperglobulinemia (8/9). Median survival time was 26.5 days (range, 0-122 days), and the disease was ultimately fatal in all 10 dogs.
The geographic distribution of pythiosis has widened in recent years to include the western United States. Factors that may have contributed to this change include altered rice-farming practices and landscape irrigation. Veterinarians in California should be familiar with the clinicopathologic features associated with GI pythiosis to aid in early diagnosis and effective treatment.

0 Followers
 · 
134 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Pythiosis is reported for the first time in a goat. The affected goat had daily access to an aquatic environment and had developed an ulcerative lesion on the skin of the left hindlimb. Microscopically, there were dermal pyogranulomas with 'negatively stained' hyphae, which were identified immunohistochemically as Pythium insidiosum. Copyright © 2014 Elsevier Ltd. All rights reserved.
    Journal of Comparative Pathology 12/2014; DOI:10.1016/j.jcpa.2014.11.005 · 1.10 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Pythiosis is an infectious disease of humans and animals living in tropical and subtropical countries. The etiologic agent is the pathogenic oomycete Pythium insidiosum. The disease has a high rate of morbidity and mortality. Patients usually presented with symptoms associated with arterial or ocular infection. Most patients underwent surgical removal of an infected organs (i.e., legs and eyes) to control the infection. Early and accurate diagnosis is important because it leads to prompt treatment and better prognosis for patients with pythiosis. Here, we developed and evaluated an immunofluorescent staining assay (IFA) for histodiagnosis of pythiosis. Rabbit anti-P. insidiosum antibodies were generated for direct detection of P. insidiosum in paraffin-embedded samples. Sixteen P. insidiosum isolates and 16 other fungi were used to prepare paraffin-embedded culture blocks for diagnostic performance evaluation of IFA. As a result, all culture blocks prepared from P. insidiosum were stained positive, while those prepared from the other fungi (control) were stained negative. IFA was further evaluated using paraffin-embedded tissue blocks prepared from infected tissues of patients with vascular pythiosis (n=3) and other fungal infections (2 Candida albicans, 1 Aspergillus flavus, and 1 Fusarium sp). All infected-tissue blocks from pythiosis patients were tested positive, while those from patients with other mycoses were tested negative. Taken together, the developed IFA provided high diagnostic performance (100% sensitivity and specificity), and could be used to facilitate diagnosis of pythiosis.
    The 6th National Science Research Conference, Faculty of Science, Burapha University; 03/2014
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: RESUMEN RESUMEN La pythiosis es una enfermedad que se presenta en humanos y en otros mamíferos, tales como equinos, caninos y bovinos. El agente etiológico de esta patología es Pythium insidiosum, un oomycete acuático presente en diversas regiones geográficas del mundo. Las manifestaciones clínicas cutáneas de tipo granulomatoso y gastrointestinales son las presentaciones que predominan en animales infectados. La infección puede diseminarse hacia otros tejidos (ej., óseo y pulmonar). La pythiosis humana tiene cuatro formas clínicas de presentación: cutánea o subcutánea, vascular, ocular y ocasionalmente se presenta en localizaciones anatómica inusuales (ej., cerebro). En Venezuela se han reportado casos en caninos, bovinos y equinos. En este artículo se presenta una revisión actualizada de la literatura en relación a la epidemiología, ciclo de vida del agente etiológico, patogénesis, presentación clínica en animales y humanos, diagnóstico, tratamiento y los casos de pythiosis en Venezuela. ABSTRACT Pythiosis is a disease that occurs in humans and other mammals, such as horses, canines and cattle. The etiologic agent is Pythium insidiosum, an aquatic oomycete present in various geographic regions of the world. The cutaneous granulomatous form and gastrointestinal are common clinical presentations which predominate in infected animals. The infection can spread to other tissues (e.g. osseous and pulmonary). Human pythiosis has four clinical forms: cutaneous or subcutaneous, vascular, ocular and occasional anatomical sites may occur (e.g.
    Salus 04/2011; 15(1):38-45.