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    ABSTRACT: B-cell activating factor (BAFF), an important immune regulatory cytokine, is involved in development of autoimmune diseases. Although BAFF is expressed in various cells, including dendritic cells and monocytes, BAFF expression on B-cells has not been well documented. In the present study, BAFF molecules on dendritic cells and naïve and memory B-cells in autoimmune bullous diseases, including pemphigus vulgaris, pemphigus foliaceus and bullous pemphigoid, were analyzed by flow cytometry. Compared to healthy controls, BAFF expression on naïve and memory B-cells increased significantly in bullous pemphigoid. No difference in BAFF receptor expression in naïve and memory B-cells was shown among all study groups. Furthermore, BAFF expression in both naïve and memory B-cells of bullous pemphigoid, but not healthy controls, was detected by confocal microscopic analysis. These results implied that BAFF expressed by B-cells may play a pathogenic role in autoimmune bullous diseases, particularly bullous pemphigoid.This article is protected by copyright. All rights reserved.
    Experimental Dermatology 04/2014; 23(8). DOI:10.1111/exd.12421 · 4.12 Impact Factor
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    ABSTRACT: Psoriasis is a chronic inflammatory skin disease with an immunogenetic background. This study aimed to determine the association between three functional SNPs of BANK1 (rs10516487, rs17266594 and rs3733197) with psoriasis in Southern Han Chinese population by determining their frequency in 242 patients with psoriasis and 317 healthy individuals. The genotype frequencies of the detected polymorphisms were analysed in relation to the susceptibility of psoriasis. Our data show that there is no significant difference in genotype distribution for the three BANK1 SNPs between patients and healthy controls. The AA frequency of rs3733197 is significantly higher in patients with psoriasis onset before the age of 23 than in those with late disease onset (P =  0.0069). In addition, analysis on BANK1 haplotype also suggests a protective role for TGC and CAT haplotype from psoriasis (OR 0.55, 95% CI: 0.34-0.89; P = 0.0144; OR 0.62, 95% CI: 0.42-0.92; P = 0.0175), whereas CGT haplotype is associated with increased risk of the disease (OR 1.38, 95% CI: 1.05-1.81, P = 0.0203). Overall, our result indicates that polymorphism in BANK1 is associated with susceptibility to psoriasis in Southern Han Chinese.
    International Journal of Immunogenetics 12/2011; 38(6):507-12. DOI:10.1111/j.1744-313X.2011.01045.x · 1.34 Impact Factor
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    ABSTRACT: B-cell-activating factor belonging to the tumour necrosis factor family (BAFF) is known for its role in the survival and maturation of B cells. It has been recently suggested that BAFF also plays important roles in T-cell activation in T-cell mediated diseases such as psoriasis. To investigate the role of BAFF in cutaneous T-cell lymphoma (CTCL). BAFF messenger RNA (mRNA) expression in skin samples (24 CTCL cases and seven healthy controls) and in skin-derived fibroblasts (five CTCL cases and five healthy controls) was examined by quantitative reverse transcription-polymerase chain reaction. We also performed immunohistochemical staining for BAFF and its receptors. Serum BAFF levels were measured in patients with CTCL (n=46), atopic dermatitis (n=36) or psoriasis (n=27) and 27 healthy controls by enzyme-linked immunosorbent assay. Lesional skin of CTCL contained higher levels of BAFF mRNA than normal skin and the expression levels correlated with disease activity. BAFF mRNA expression levels were elevated in fibroblasts from CTCL skin. Tumour cells in the lesional skin of CTCL expressed BAFF and its receptors, while fibroblasts expressed only BAFF. Serum BAFF levels of CTCL patients were significantly higher than those of healthy controls and correlated with types of skin lesions and clinical stages. They also significantly correlated with serum soluble interleukin-2 receptor and lactate dehydrogenase levels. BAFF expression in CTCL skin and serum BAFF levels are significantly increased and correlate with the severity of CTCL. These results suggest that BAFF may have important roles in the development of CTCL.
    British Journal of Dermatology 04/2012; 167(2):359-67. DOI:10.1111/j.1365-2133.2012.10998.x · 3.76 Impact Factor