Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo.
ABSTRACT Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal jelly proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant activity against lipid peroxidation (LPO, p<0.001), than did water-soluble royal jelly protein (WSRJP) in vitro. We also investigated the in vivo antioxidant activity of RJPx against ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA group (Group Fe), and an Fe-NTA with RJPx group (Group Fe+R). Rats in Group Fe+R were fed RJPx (2 g/kg body weight) daily for 5 wk. Fe-NTA (8 mg Fe/kg body weight) was then intraperitoneally injected, and serum lipid levels were examined 2 h later. Serum total cholesterol (TC) levels were lower (p<0.05) while low-density lipoprotein (LDL) and LPO were significantly higher (p<0.01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo.
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ABSTRACT: Hypercholesterolaemia (HPC) is a risk factor of cardiovascular disease. Synthetic medicines cause serious side effects that cause an imbalance in the body’s functions. Therefore utilization of natural compounds could be an alternative concept in the treatment of diseases, as they have no side effects on human health. The present work is designed to evaluate the immunomodulatory role of royal jelly (RJ) on the aorta in hypercholestrolaemic rats. Cholesterol (30 mg/kg/day) administration for two months caused a significant increase of total cholesterol, triglycerides, LDL, CD4 and CD8 in serum and diminution in HDL levels. Aortic histopathological lesions are represented by deposition of fats, loss of smooth muscle fibres and an increase in CD3, CD86 and eNOS expression in the tunica intima. RJ administration (300 mg/kg/day) with CH, produced a counteractive effect, represented in recompense of biochemical alteration, CD4 and CD8 expression. RJ intake also amended the histological picture. The immunohistochemical picture revealed a decrease in CD3, CD86 and eNOS in the aortic tissue. These findings attributed to the significant immunomodulatory effect of RJ remedy suppress deleterious effects of hypercholesterolaemia.The Journal of Basic & Applied Zoology. 08/2014;
- Iranian Journal of Reproductive Medicine 01/2015; 13(1):15-22. · 0.19 Impact Factor
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ABSTRACT: ABSTRACT Background: An adverse effect of oxymetholone (OXM), an anabolic-androgenic steroid used as energetic medicine, is reproductive toxicity. Royal jelly (RJ) is an efficient antioxidant that has been used to treat reproductive problems. In this study, we investigated the effects of RJ on OXM-induced oxidative injuries in mouse testes. Methods: Male mice were divided into four groups. Two groups of mice were administered OXM (5 mg/kg/day, p.o.) for 28 days. One of these groups received RJ (100 mg/kg/day, p.o.) concurrently. A vehicle-treated control group and a RJ control group were also included. Results: The OXM-treated group showed a significant decrease in the serum testosterone concentration and spermatogenic activities, along with many histological alterations. OXM treatment also caused a significant decrease in catalase activity with an increase in lipid peroxidation in the mouse testes. The above-noted parameters were restored to near normal levels by RJ co-administration. Conclusion: The results demonstrate that RJ protects against OXM-induced reproductive toxicities. Keywords: Mouse, Oxymetholone, Royal Jelly, Testis.Iranian Journal of Toxicology. 06/2014; 8(25):1073-1080.