Royal Jelly Peptides Inhibit Lipid Peroxidation In Vitro and In Vivo
Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal jelly proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant activity against lipid peroxidation (LPO, p<0.001), than did water-soluble royal jelly protein (WSRJP) in vitro. We also investigated the in vivo antioxidant activity of RJPx against ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA group (Group Fe), and an Fe-NTA with RJPx group (Group Fe+R). Rats in Group Fe+R were fed RJPx (2 g/kg body weight) daily for 5 wk. Fe-NTA (8 mg Fe/kg body weight) was then intraperitoneally injected, and serum lipid levels were examined 2 h later. Serum total cholesterol (TC) levels were lower (p<0.05) while low-density lipoprotein (LDL) and LPO were significantly higher (p<0.01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo.
Available from: polipapers.upv.es
- "In addition, adding RJ to mice for 16 wk, the levels of 8-hydroxy-2- deoxyguanosine (an oxidative stress marker) were significantly reduced in kidney and serum (Inoue et al., 2003). It has also been shown to inhibit lipid peroxidation both in vitro and in vivo (Hang et al., 2008). "
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ABSTRACT: To alleviate the deleterious effect of heat stress during summer conditions on male rabbits' reproduction, 40 V Line adult rabbit bucks (on av. 8 mo old) were divided into 4 experimental groups and exposed to temperatures ranging from 23 to 36° C. Bucks in the 1st, 2nd, 3rd, and 4thgroup were supplemented with 0, 50, 100 or 150 mg of Chinese royal jelly (RJ)/kg twice per week, respectively, over a 20-wk period. Semen quality and blood biochemical constituents were evaluated. RJ at any dose exhibited a significant increase (P<0.05) in rabbits' sperm concentration, total sperm output, sperm motility, live sperm and normal sperm compared to the untreated controls. Plasma total protein, albumin, globulin, glucose and high density lipids (HDL) concentrations were significantly (P<0.05) boosted in the RJ groups compared to the controls. In contrast, RJ treatment resulted in a significant (P<0.05) reduction in plasma total lipids, triglycerides, cholesterol and low density lipids (LDL) concentrations. Treatment with RJ significantly boosted (P<0.05) testosterone concentration in the RJ groups to reach 110, 120 and 128%, respectively, of the control group. Improved kidney and liver functions were observed in the RJ bucks groups where plasma creatinine, urea concentrations, aspartate aminotransferase and alanine aminotransferase enzyme activities were significantly (P<0.05) decreased by RJ treatments. Treating bucks subjected to heat stress by different RJ doses increased (P<0.05) total antioxidant capacity to 106, 111 and 115% of basal, but significantly reduced (P<0.05) malondialdehyde and thiobarbituric acid reactive substances compared to the untreated. It was concluded that Chinese royal jelly supplementation for heat-stressed male rabbits can counteract summer infertility and improve their physiological status.
World Rabbit Science 09/2014; 22(3):241. DOI:10.4995/wrs.2014.1677 · 0.82 Impact Factor
- "Recently, studies have suggested that RJ has free-radical scavenging capacity and is a highly efficient antioxidant. RJ has been shown that inhibits lipid proxidation both in vitro and in vivo. "
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ABSTRACT: It has been recently demonstrated that Royal jelly (RJ) has a beneficial role on neural functions. Alzheimer's disease (AD) is associated with impairments of learning and memory. Therefore, the present study was designed to examine the effect of RJ on spatial learning and memory in rats after intracerebroventricular injection of streptozotocin (icv-STZ).
Rats were infused bilaterally with an icv injection of STZ, while sham rats received vehicle only. The rats were feed with RJ-contained food (3% w/w) (lyophilized RJ mixed with powdered regular food) or regular food for 10 days. Then spatial learning and memory was tested in the rats by Morris water maze test.
Results showed that in icv-STZ group latency and path length were increased as compared to sham group, also icv-STZ rats less remembered the target quadrant that previously the platform was located; however, these were protected significantly in STZ group that received RJ-containing food.
Our findings support the potential neuroprotective role of RJ and its helpful effects in AD.
07/2012; 1:26. DOI:10.4103/2277-9175.98150
Available from: Samar A Elnagar
- "It also contains many trace minerals, some enzymes, antibacterial and antibiotic components, and trace amounts of vitamin C. Vitamins A, D, E and K are completely absent from RJ (Graham, 1992). RJ has been used in animal research, with many benefits; it was shown to protect rats against mutagenic effects of adriamycin and g radiation (El-Fiky et al., 2008), inhibits lipid peroxidation both in vitro and in vivo (Hang et al., 2008), shows cardioprotective characteristics in myocardial ischemia rats (Krylov et al., 2006), has immunomodulatory activities (Gasic et al., 2007), and it was proven that its antioxidant properties may play an important role on its effects on aging process and stress reactions in rats (Ikeda et al., 1996). This study was conducted to evaluate the ability of RJ feeding to reduce physiological strain of broiler rabbits exposed to Egyptian summer conditions. "
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ABSTRACT: Exposure of growing rabbits to heat stress during summer adversely affects their performance leading to major production losses. A total number of 48 rabbits, unsexed V-line weaned rabbits, were randomly divided into four experimental groups, temperature ranged from high at 32°C to low at 23°C. Animals of the 2nd, 3rd and 4th group were individually orally given 200, 400 or 800 mg royal jelly (RJ)/kg BW once a week, respectively, to evaluate RJ ability to reduce physiological strain resulted from heat stress. Weekly BW gain increased by 10.4, 11.8 and 10.8%, and feed conversion ratio was significantly improved by 20, 24 and 18% with RJ treatments. Serum total protein, albumin and globulin increased, whereas serum total lipids, cholesterol and triglycerides decreased with RJ treatments. Creatinine was reduced by 21, 30 and 18% and uric acid by 14, 25 and 18% compared with the heat stressed control with the three doses of RJ. Glucose level increased significantly to reach 116, 125, and 120% of heat stressed control. Calcium, phosphorus and alkaline phosphatase increased significantly with RJ treatments indicating the occurrence of active bone deposition. Thyroid hormone levels increased significantly to reach 108, 111, and 112% of heat stressed control rabbits with the three doses of RJ, counteracting the hypothyroid state resulted from heat stress. It can be concluded that RJ administration to heat stressed growing rabbits can reduce physiological strain resulted from heat stress.
animal 09/2010; 4(9):1547-52. DOI:10.1017/S1751731110000753 · 1.84 Impact Factor
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