Pseudomonas duriflava sp. nov., isolated from a desert soil.
ABSTRACT A Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain HR2(T), was isolated from a soil sample from the Taklimaken Desert in Xinjiang Province, China. Strain HR2(T) grew optimally at pH 7.0-8.0 and 30-37 degrees C in the presence of 0-1 % (w/v) NaCl. An analysis of 16S rRNA gene sequences revealed that strain HR2(T) fell within the radiation of the genus Pseudomonas, the highest level of similarity being found with respect to Pseudomonas luteola IAM 13000(T) (97.5 %); the levels of sequence similarity with respect to other recognized Pseudomonas species were <96.4 %. DNA-DNA hybridization showed that the genetic relatedness between strain HR2(T) and P. luteola IAM 13000(T) was 53.2 %. The G+C content of the genomic DNA of strain HR2(T) was 55.2 mol%. The major fatty acids were 18 : 1, summed feature 3 and 16 : 0. The hydroxylated fatty acids 10 : 0 3-OH, 12 : 0 3-OH and 12 : 0 2-OH were also present. The data obtained in this polyphasic study indicated that this isolate represents a novel species of the genus Pseudomonas, for which the name Pseudomonas duriflava sp. nov. is proposed. The type strain is HR2(T) (=KCTC 22129(T)=CGMCC 1.6858(T)).
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ABSTRACT: An ad hoc committee for the re-evaluation of the species definition in bacteriology met in Gent, Belgium, in February 2002. The committee made various recommendations regarding the species definition in the light of developments in methodologies available to systematists.International journal of systematic and evolutionary microbiology 06/2002; 52(Pt 3):1043-7. · 2.11 Impact Factor
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ABSTRACT: A nitrogen-fixing bacterium, designated strain 6H33b(T), was isolated from a compost pile in Japan. The nitrogenase activity of this strain was detected based on its acetylene-reducing activity under low oxygen concentrations (2-4%). An analysis of the genes responsible for nitrogen fixation in this strain, nifH and nifD, indicated a close relationship to those of Pseudomonas stutzeri A15 (A1501). Sequence similarity searches based on the 16S rRNA gene sequences showed that strain 6H33b(T) belongs within the genus Pseudomonas sensu stricto; closest similarity was with Pseudomonas indica (97.3%). A comparison of several taxonomic characteristics of 6H33b(T) with those of P. indica and some type strains of the genus Pseudomonas sensu stricto indicated that 6H33b(T) could be distinguished from P. indica based on the presence of nitrogen fixation ability, the absence of nitrate reduction and denitrification abilities and the utilization of some sugars and organic acids. Phylogenetic analyses and the results of DNA-DNA hybridization experiments also indicated that strain 6H33b(T) represents a species distinct from P. indica. From these results, it is proposed that strain 6H33b(T) (=ATCC BAA-1049(T)=JCM 12708(T)) is classified as the type strain of a novel species of the genus Pseudomonas sensu stricto under the name Pseudomonas azotifigens sp. nov.International journal of systematic and evolutionary microbiology 08/2005; 55(Pt 4):1539-44. · 2.11 Impact Factor
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ABSTRACT: The complete nucleotide sequence of the 16S RNA gene from the rrnB cistron of Escherichia coli has been determined by using three rapid DNA sequencing methods. Nearly all of the structure has been confirmed by two to six independent sequence determinations on both DNA strands. The length of the 16S rRNA chain inferred from the DNA sequence is 1541 nucleotides, in close agreement with previous estimates. We note discrepancies between this sequence and the most recent version of it reported from direct RNA sequencing [Ehresmann, C., Stiegler, P., Carbon, P. & Ebel, J.P. (1977) FEBS Lett. 84, 337-341]. A few of these may be explained by heterogeneity among 16S rRNA sequences from different cistrons. No nucleotide sequences were found in the 16S rRNA gene that cannot be reconciled with RNase digestion products of mature 16S rRNA.Proceedings of the National Academy of Sciences 10/1978; 75(10):4801-5. · 9.74 Impact Factor