Brucella canis Isolates from Canadian Dogs.

The Canadian veterinary journal. La revue veterinaire canadienne (Impact Factor: 0.47). 02/1988; 29(2):149-52.
Source: PubMed

ABSTRACT Eleven Brucella canis isolates from Canadian dogs were characterized by dye and antibiotic sensitivity, phage susceptibility, urease and H(2)S production, CO(2) requirement, and reaction with monospecific A,M, and R anti-Brucella antiserum. The isolates could be separated into two distinct groups. One group had a sensitivity pattern similar to that seen with the American type strain RM666, while the other group had a pattern identical to that of a Mexican strain, Mex 51. Epidemiological studies supported contraction of infections in the United States and Mexico respectively. The characteristics of all isolates were stable after repeated subculture indicating that strain differences could serve as useful epidemiological markers and supporting division of the species into two biovars.

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    ABSTRACT: This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.
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    Brazilian Journal of Microbiology 01/2004; 35. DOI:10.1590/S1517-83822004000100027 · 0.45 Impact Factor
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    ABSTRACT: This study was carried out to determine the incidence of Brucella canis in male dogs with reproductive problems. Fifty-six dogs were chosen from the patient population brought to the Small Animal Hospital of the College of Veterinary Medicine, at the National Autonomous University of Mexico. For the clini- cal reproductive evaluation, semen was collected and a rapid agglutination test and blood culture were performed, as well as a semen culture. Forty sera samples were collected from animals with reproductive problems, eleven from dogs with clinically related problems and 5 from dogs that had a combination of both. Semen was collected by masturbation from 41 of the 56 dogs. Of the 56 dogs, 24 (42.8%) were posi- tive for agglutination test, 3 (5.3%) were positive in blood cultures, and all semen cultures were negative. The main reproductive problems in seropositive dogs were: epididymitis, orchitis and azoospermia. In the blood cultured positive animals, the problems were testicular atrophy, orchitis and epididymitis. In cases that were positive to serology and blood culture, diskospondylitis was present.


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